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pBacMam2-DiEx-LIC_LRRK2:2141-2527

Plasmid

#210900

Purpose

Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:T2141-E2527 (LRRK2 Human)

Tags

AENLYFQSHHHHHHHHHHDYKDDDDK and M

Expression

Insect

Mutation

M2397T natural variant ( VAR_024965)

Promoter

P10 and CMV

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_CDC40:236-578

Plasmid

#210885

Purpose

Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC40:E236-W578 (CDC40 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pR5-28

Plasmid

#22289

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Expression

Yeast

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
pVAM07

Plasmid

#252057

Purpose

Hygromycin B resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM05

Plasmid

#252056

Purpose

Gentamicin resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM03

Plasmid

#251701

Purpose

Tetracycline resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGFP-ATG13(315)

Plasmid

#122072

Purpose

Expressing GFP-Atg13

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAW12

Plasmid

#110224

Purpose

integration of ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pR5-30

Plasmid

#22290

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Expression

Yeast

Mutation

D681, E682->AA

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
piHGpd/P82

Plasmid

#1325

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
TaHKT1_pYES2

Plasmid

#244447

Purpose

High-copy episomal vector for galactose-inducible expression of TaHKT1 protein in Saccharomyces cerevisiae.

Depositor

Julian Schroeder

Article

Schachtman et al Nature. 1994 Aug 25;370(6491):655-8. doi

Insert

TaHKT1

Expression

Yeast

Promoter

T7 promoter

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sgd1

Plasmid

#100000

Purpose

Shuttle Sgd1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sgd1 (SGD1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Bms1

Plasmid

#100001

Purpose

Shuttle Bms1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Bms1 (BMS1 Budding Yeast)

Expression

Yeast

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb1

Plasmid

#99981

Purpose

Shuttle yeast Ckb1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb1 (CKB1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb2

Plasmid

#99982

Purpose

Shuttle yeast Ckb2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb2 (CKB2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp11

Plasmid

#99984

Purpose

Shuttle yeast Utp11 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp11 (UTP11 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Pfa1

Plasmid

#99999

Purpose

Shuttle yeast Pfa1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Pfa1 (SQS1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Cka1

Plasmid

#99979

Purpose

Shuttle yeast Cka1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka1 (CKA1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CF-Ins54p

Plasmid

#20155

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (INP54 Budding Yeast)

Expression

Mammalian

Mutation

Stop codon at position 331.

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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KBB464

Plasmid

#185097

Purpose

HA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminus

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

Pom152DGlyc-GFP

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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