We narrowed to 42,599 results for: cha

1. pAC8_MF (VE5596)

   Plasmid

   #139768

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   PH or p10

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS306Sup35R1

   Plasmid

   #17715

   Depositor

   Susan Lindquist

   Article

   Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

   Insert

   pRS306Sup35R1 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   An pRS306 based integrating plasmid designed for …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

   Plasmid

   #153446

   Purpose

   E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)

   Plasmid

   #106465

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)

   Plasmid

   #106471

   Purpose

   Expresses Prc1 with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   PRC1
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   PRC1

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPicZ:mTREK-1_cryst_I110D

   Plasmid

   #209640

   Purpose

   P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

   Depositor

   Dan Minor

   Article

   Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
   

   Use

   Tags

   SNS- 3C_cleavage_site-TAAA-GFP

   Expression

   Yeast

   Mutation

   aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFPEasy9R

   Plasmid

   #218291

   Purpose

   Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ShadowY-CLC

   Plasmid

   #193560

   Purpose

   Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   ShadowY

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CLC-ShadowY

   Plasmid

   #193561

   Purpose

   Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   ShadowY

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTSK405

    Plasmid

    #190817

    Purpose

    For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

    Depositor

    Tatiana Karpova, Gunjan Mehta

    Article

    Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

    Insert

    URA3 (URA3 Budding Yeast)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

    Plasmid

    #115432

    Purpose

    Vector for expressing RPL28 with mutagenized exon2

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    exon 2 deleted, with MfeI / NheI cloning site

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)

    Plasmid

    #106461

    Purpose

    Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W
    

    Use

    Tags

    PA

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

    Plasmid

    #106470

    Purpose

    Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W
    

    Use

    Tags

    PA

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSP64-hERG1a D540K

    Plasmid

    #53057

    Purpose

    Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Aspartate 540 to Lysine

    Promoter

    SP6

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES:hTRAAK(W262S)

    Plasmid

    #133078

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES:hTRAAK(G124I)

    Plasmid

    #133079

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G124I

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mEGFP-CaMKIIa (T286D)

    Plasmid

    #127391

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid

    #127390

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge