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Flag-HA-USP18

Plasmid

#22572

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP18 (USP18 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-myc3-bTrCP

Plasmid

#20718

Depositor

Yue Xiong

Publication

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

beta-transducin repeat containing (BTRC) (Btrc Mouse)

Tags

Myc3

Expression

Mammalian

Mutation

*

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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T4 Lysozyme WT*

Plasmid

#18111

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Mutation

No cysteines: C54T, C97A

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Flag-mPIAS1

Plasmid

#15206

Depositor

Ke Shuai

Publication

Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

Insert

PIAS1 (Pias1 Mouse)

Tags

Flag

Expression

Mammalian

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p2xFLAGhYAP1

Plasmid

#17791

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pWZL hygro Flag HA TRAP220 wt

Plasmid

#17433

Purpose

Retroviral expression of Med1/TRAP220 in mammalian cells

Depositor

Kai Ge

Publication

Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Insert

TRAP220 (MED1 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMXs-Klf4-IP

Plasmid

#15920

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLKO.1-TSC2

Plasmid

#15478

Depositor

Do-Hyung Kim

Publication

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

TSC2 shRNA (TSC2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Publication

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LentiGuide Puro-P2A-EGFP

Plasmid

#137729

Purpose

For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

Depositor

Fredrik Wermeling

Publication

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

EGFP

Expression

Bacterial and Mammalian

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Publication

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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lentiSAM v2 (Puro)

Plasmid

#92062

Purpose

Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Adam Karpf

Publication

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA) and EF1a (dCas9-VP64)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHAGE-E-cadherin-RFP

Plasmid

#79603

Purpose

pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

Depositor

Jeffrey Rosen

Publication

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

CDH1 promoter region (CDH1 Human)

Use

Lentiviral

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Publication

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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nls-EGFP

Plasmid

#67652

Purpose

Mammalian expression vector driving nls-EGFP.

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

nls-EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PP7_mCherry

Plasmid

#61763

Purpose

Expresses PP7 coat protein fused to mCherry

Depositor

Daniel Larson

Publication

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

PP7 coat protein fused to mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Ubc promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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