4,235 results

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Str-Golgin84_VSVG-SBP-EGFP

Plasmid

#65305

Purpose

synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC wt

Plasmid

#19304

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact-tdTomato

Plasmid

#64048

Purpose

Fluorescent reporter for F-actin labeling in living cells

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Lifeact

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-dsRedExpress

Plasmid

#65032

Purpose

GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

dsRedExpress

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AIO-Puro

Plasmid

#74630

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Puromycin resistant marker

Expression

Mammalian

Promoter

Cbh

Available Since

May 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Ub-R-GFP

Plasmid

#11939

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-R-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pLifeAct-mTurquoise2

Plasmid

#36201

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

Plasmid

#55639

Purpose

Flp-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Oct3/4

Plasmid

#13366

Purpose

Retroviral expression of mouse Oct3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSL2680

Plasmid

#85581

Purpose

Replicating base vector for constructing cpf1/CRISPR editing plasmids

Depositor

Himadri Pakrasi

Article

Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9

Plasmid

#47106

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_Donor (EMM0021)

Plasmid

#63934

Purpose

3X Flag with P2A-Neo for 3' tagging of endogenous proteins

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Glycine-Serine-3XFlag-P2A-NeoR

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5-Smad7-HA

Plasmid

#11733

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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HA-DDX3

Plasmid

#44975

Depositor

Robin Reed

Article

Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

Insert

DDX3 (DDX3X Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 wt

Plasmid

#16434

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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