We narrowed to 4,498 results for: 187

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p8189 pHAGE N-V5 PTPN14 C1121S

Plasmid

#221648

Purpose

Constitutive ectopic expression of human PTPN14 C1121S mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 C1121S (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

C1121S mutation at the putative catalytic site

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEY50

Plasmid

#223443

Purpose

mbr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBF2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mbr-1 (mbr-1 Nematode)

Expression

Worm

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-λN-HA-NOT1 isoform C (JM155)

Plasmid

#206449

Purpose

Not1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Not1 (Not1 Fly)

Tags

lambda N HA

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT1 isoform C (HK53)

Plasmid

#206440

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT2 isoform A (HK54)

Plasmid

#206441

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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HA-RIPLET ΔPRY/SPRY

Plasmid

#218516

Purpose

RIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta PRY/SPRY domain (RNF135 Human)

Expression

Mammalian

Available Since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD2 CTF-FLAG

Plasmid

#217688

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRD2 (ADGRD2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-636

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBC160

Plasmid

#202248

Purpose

Level 2 for strain-specific barcode DNA tag with bc187 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc187

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide2 in pX458

Plasmid

#211536

Purpose

sgRNA-2 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide1 in pX458

Plasmid

#211535

Purpose

sgRNA-1 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hPRL2_1-163

Plasmid

#209794

Purpose

Bacterial expression of human PRL-2 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-2 (PTP4A2 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-163 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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acvr1l-VAAA-HA

Plasmid

#201905

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Mutation

T186V, S187A, S189A, S191A

Promoter

SP6

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3B DD 593-747

Plasmid

#193932

Purpose

Mammalian expression of GFP-tagged Kif 3B DD 593-747

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3B (Kif3b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-DmNot1_1710-2505_R

Plasmid

#147440

Purpose

Bacterial Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1710-2505 (Not1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-dsRNAres_N

Plasmid

#147029

Purpose

Insect Expression of DmNot1-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmNot1-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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