We narrowed to 4,547 results for: BRE

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pEGFP-Flag-stm2585_Y167F

Plasmid

#174398

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Y167F

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585-gp130

Plasmid

#174393

Purpose

Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Tags

Flag

Expression

Mammalian

Mutation

140-177 of sarA replaced with homologous IL6ST se…

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585_Y167F

Plasmid

#174392

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Mutation

Tyr 167 changed to Phe

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK2 3'UTR KGA

Plasmid

#110402

Purpose

Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pX330.puro sgRNA KGA Stop1

Plasmid

#110405

Purpose

CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

Plasmid

#110408

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Promoter

none

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-OgNLuc

Plasmid

#135951

Purpose

Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph18

Plasmid

#133029

Purpose

Expresses Xph18 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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AcrIIC1-mCherry chimera #10

Plasmid

#128113

Purpose

Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrIIC1-mCherry chimera #10

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph15

Plasmid

#133030

Purpose

Expresses Xph15 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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A3H HapII R175/6E-Cas9n-UGI

Plasmid

#119142

Purpose

Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI

Use

CRISPR

Mutation

A3H Hap II RR175/6EE

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG SMAD4

Plasmid

#83094

Purpose

Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

SMAD4 (SMAD4 Human)

Use

Gateway Cloning

Tags

3xFLAG

Promoter

TRE tight promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
AAV CMV-driven AcrIIA4-2xmiR-1 target sites

Plasmid

#120299

Purpose

AAV vector for expression of AcrIIA4 with two miR-1 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4-2xmiR-1 binding sites

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

Plasmid

#120300

Purpose

AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSL5069 (pDonor(AAVS1),CRISPR_Pse)

Plasmid

#200904

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2

Plasmid

#85208

Purpose

Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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YAP/TAZ tdTomato TEAD Binding Reporter

Plasmid

#228554

Purpose

Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

Depositor

Cole DeForest

Article

Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

Insert

tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

GTIIC TEAD-Binding Responsive Element

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-EGFP BlastR

Plasmid

#84383

Purpose

Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
HA-Rab11-WT

Plasmid

#101047

Purpose

Expresses HA-tagged human Rab11-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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