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AAV-Best1-sCX3CL1

Plasmid

#164422

Purpose

AAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epithelium

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

Cx3cl1 (Cx3cl1 Mouse)

Use

AAV

Expression

Mammalian

Mutation

deleted terminal 59 amino acids

Promoter

Best1

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_2

Plasmid

#155088

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2-F75A

Plasmid

#196862

Purpose

Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2-F75A (Nedd1 Rat)

Expression

Mammalian

Mutation

F75A in the CM1 domain

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pNK497

Plasmid

#219754

Purpose

MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH

Expression

Plant

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1

Plasmid

#237678

Purpose

For overexpression of mEGFP-SS18-SSX1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1 (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDY0207 ACTB atgRNA with v1 scaffold

Plasmid

#179107

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA

Use

CRISPR

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-kz-CD20-Puro

Plasmid

#209759

Purpose

Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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IronFistMUT.

Plasmid

#243008

Purpose

Iron binding deficient IronFist

Depositor

Roland Malli

Article

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Inserts

hemerythrin-like-mNeonGreen (FBXL5 Human)
T2A
mCherry

Use

Gateway

Tags

mNeonGreen

Expression

Mammalian

Mutation

H15A, H57A, E58A, E61A, H126A, E130A

Promoter

human phosphoglycerate kinase (hPGK)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDY0310 TP901-1 Integrase

Plasmid

#179110

Purpose

TP901-1 Integrase expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

TP901-1 Integrase

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pINDUCER22-MKK6E-Flag

Plasmid

#99259

Purpose

Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

Depositor

Alejandro Adam, Peter Vincent

Article

Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

Insert

MKK6E (MAP2K6 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S207E T211E

Promoter

TRE2

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-CD20-Puro

Plasmid

#209757

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-EB3-mNeonGreen

Plasmid

#196848

Purpose

labelling of microtubule plus ends

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EB3-NeonGreen (MAPRE3 Human)

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-XL-rAkap9

Plasmid

#196867

Purpose

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akap9 (Akap9 Rat)

Expression

Bacterial

Promoter

Lac promoter and T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-fCX3CL1

Plasmid

#164421

Purpose

AAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptors

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

Cx3cl1 (Cx3cl1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CB1-HIF2a-GFP-T2A-Puro

Plasmid

#71708

Purpose

Lentiviral expression of HIF2A-GFP

Depositor

Eric Jonasch, Xiande Liu

Article

Liu et al Oncogene. 2015 May 7;34(19):2450-60. doi

Insert

HIF2A (EPAS1 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_AAVS1_sgRNA_2

Plasmid

#155105

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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