We narrowed to 5,173 results for: pcDNA3.1+

1. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag1-3HABeta2-mEos2

   Plasmid

   #114187

   Purpose

   studying clustering of Beta2 receptors by superresolution microscopy (PALM)

   Depositor

   Aleksandra Radenovic

   Article

   Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

   Insert

   Adrenergic receptor beta 2 (ADRB2 Human)
   

   Use

   Tags

   3HA, FLAG, and mEOS2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EcPPK1

   Plasmid

   #108850

   Purpose

   Expresses E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

   Insert

   Polyphosphate Kinase 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-HCMVgL-FLAG (Merlin)

   Plasmid

   #136450

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein L (UL115 Human betaherpesvirus 5)
   

   Use

   Tags

   Short SGGSG linker and FLAG tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dr-EGFR

   Plasmid

   #184004

   Purpose

   Dr-EGFR expression in mammalian cells

   Depositor

   Vladislav Verkhusha

   Article

   Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

   Insert

   Dr-EGFR (EGFR Human)
   

   Use

   Tags

   Deinococcus radiodurans bacterial phytochrome

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-ERK2 L198A L232A

   Plasmid

   #8978

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L198A, L232A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

7. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 PBD wt (Nigg AH12)

   Plasmid

   #41162

   Depositor

   Erich Nigg

   Article

   Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

   Insert

   PBD (PLK1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Contains Plk1-PBD only (aa 326–603)

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGP-CMV-jGCaMP8f F366H

   Plasmid

   #204141

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Use

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pER-eZinCh-2

    Plasmid

    #70009

    Purpose

    expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    ER-eZinCh-2
    

    Use

    Tags

    KDEL sequence and PPI signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid

    #188993

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    D201G, A258T, K327R, G328S, G381V, G429D, T450A

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-YL1

    Plasmid

    #15333

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

    Insert

    transcription factor-like 1 (VPS72 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA_DiCre_60.F2

    Plasmid

    #60208

    Purpose

    Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
    

    Use

    Tags

    FRAP and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 2021-2112 of FRAP with a T2098L mutat…

    Promoter

    CMV

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Bongwoori

    Plasmid

    #63720

    Purpose

    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    Bongwoori
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA-SCW-HHL

    Plasmid

    #190634

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VfAU1-LOV_P2A_casp9

    Plasmid

    #131224

    Purpose

    Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    VfAU1-LOV-HA_P2A_myc-casp9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169591

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-HA-CAML

    Plasmid

    #22284

    Depositor

    Erik Lillehoj

    Article

    Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

    Insert

    calcium-modulating ligand (CAMLG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

27. b2-TS3

    Plasmid

    #103877

    Purpose

    full-length human b2 with tension sensor at b2_733

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-NES-ZapCY2

    Plasmid

    #59016

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

    Insert

    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

29. aL-mEos3.2

    Plasmid

    #113154

    Purpose

    constrained LFA-1-mEos3.2 fusion

    Depositor

    Timothy Springer

    Article

    Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

    Insert

    full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
    

    Use

    Tags

    mEOS3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. deltaPYD-NLRP11.MYC

    Plasmid

    #131197

    Purpose

    mammalian expression

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    deltaPYD-NLRP11 (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deletion of the pyrin domain (amino acids 1-124 m…

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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