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We narrowed to 8,387 results for: BLI;

Showing: 4921 - 4960 of 8387 results

  1. pHH0103 PDZK10 WW domain

    Plasmid
    #104285
    Purpose
    Bacterial expression of WW domain from PDZK10
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PDZK10 WW domain (FRMPD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104285-plasmid-map-sequence-id-202571

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  2. pHH0103 ARHGAP9 WW domain

    Plasmid
    #104286
    Purpose
    Bacterial expression of WW domain from ARHGAP9
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ARHGAP9 WW domain (ARHGAP9 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104286-plasmid-map-sequence-id-203478

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  3. pHH0103 IQGAP3 WW domain

    Plasmid
    #104289
    Purpose
    Bacterial expression of WW domain from IQGAP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    IQGAP3 WW domain (IQGAP3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104289-plasmid-map-sequence-id-203675

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  4. pHH0103 WWTR1 WW domain

    Plasmid
    #104261
    Purpose
    Bacterial expression of WW domain from WWTR1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWTR1 WW domain (WWTR1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104261-plasmid-map-sequence-id-203726

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  5. pHH0103 FNBP4 WW domain #1

    Plasmid
    #104263
    Purpose
    Bacterial expression of WW domain #1 from FNBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP4 WW domain #1 (FNBP4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104263-plasmid-map-sequence-id-203488

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  6. pHH0103 FNBP4 WW domain #2

    Plasmid
    #104264
    Purpose
    Bacterial expression of WW domain #2 from FNBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP4 WW domain #2 (FNBP4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104264-plasmid-map-sequence-id-203718

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  7. pHH0103 DRP2 WW domain

    Plasmid
    #104270
    Purpose
    Bacterial expression of WW domain from DRP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    DRP2 WW domain (DRP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104270-plasmid-map-sequence-id-202573

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  8. hC73S-pEGFP-C1

    Plasmid
    #111540
    Purpose
    Expresses human KCNB1 C73S in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73S-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73S
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111540-plasmid-map-sequence-id-217175

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  9. hC73D-pEGFP-N1

    Plasmid
    #111534
    Purpose
    Expresses human KCNB1 C73D in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73D-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73D
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111534-plasmid-map-sequence-id-217173

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  10. hC73A-DN-HA

    Plasmid
    #111531
    Purpose
    Expresses a conducting dominant neg in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human C73A-W369C-Y384T-Kv2.1 + HA (KCNB1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C73A-W369C-Y384T
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111531-plasmid-map-sequence-id-270739

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  11. hC73A-pEGFP-C1

    Plasmid
    #111539
    Purpose
    Expresses human KCNB1 C73A in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73A-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73A
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111539-plasmid-map-sequence-id-211629

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  12. hC73W-pEGFP-N1

    Plasmid
    #111537
    Purpose
    Expresses human KCNB1 C73W in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73W-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73W
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111537-plasmid-map-sequence-id-211625

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  13. hC73M-pEGFP-N1

    Plasmid
    #111535
    Purpose
    Expresses human KCNB1 C73M in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73M-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73M
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111535-plasmid-map-sequence-id-211630

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  14. His8:MBP-tev-Leu-eΔK-Flv

    Plasmid
    #83397
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83397-plasmid-map-sequence-id-175072

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  15. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

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  16. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

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  17. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

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  18. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

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  19. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

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  20. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid
    #87152
    Purpose
    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-zwf guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87152-plasmid-map-sequence-id-174941

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  21. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

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  22. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid
    #87147
    Purpose
    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-gapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87147-plasmid-map-sequence-id-174935

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  23. pJM103: 150bp Hps2 RFP on pCM66T

    Plasmid
    #74744
    Purpose
    pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pHps2, trimmed (extracted from Methylovorus gluco…
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74744-plasmid-map-sequence-id-141459

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  24. pUC57-sgDedd-2

    Plasmid
    #74436
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 4
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  26. pJK520

    Plasmid
    #72479
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a and none
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72479-plasmid-map-sequence-id-132532

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  27. pJK559

    Plasmid
    #71603
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71603-plasmid-map-sequence-id-131677

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  28. pJK562

    Plasmid
    #71604
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71604-plasmid-map-sequence-id-131680

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  29. pJK604

    Plasmid
    #71610
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71610-plasmid-map-sequence-id-131686

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  30. pJK664

    Plasmid
    #71613
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71613-plasmid-map-sequence-id-131691

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  31. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  32. pJK563

    Plasmid
    #71689
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71689-plasmid-map-sequence-id-131681

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  33. pJK508

    Plasmid
    #71598
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71598-plasmid-map-sequence-id-131672

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  34. pPyCAG-Nanog(Q124A)-IP

    Plasmid
    #62993
    Purpose
    Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q124A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 124 (125 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62993-plasmid-map-sequence-id-107891

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  35. pPyCAG-Nanog(M125A)-IP

    Plasmid
    #62994
    Purpose
    Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog M125A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Methionine 125 (126 AA in Mouse Protein) …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62994-plasmid-map-sequence-id-107893

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  36. pPyCAG-Nanog(Q138A)-IP

    Plasmid
    #62997
    Purpose
    Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q138A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 138 (139 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62997-plasmid-map-sequence-id-104599

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  37. pPyCAG-Nanog(T141A)-IP

    Plasmid
    #62999
    Purpose
    Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog T141A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Threonine 141 (142 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62999-plasmid-map-sequence-id-104602

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  38. pPyCAG-Nanog(Q144A)-IP

    Plasmid
    #63000
    Purpose
    Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q144A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 144 (145 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63000-plasmid-map-sequence-id-104604

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  39. pPyCAG-Nanog(N145A)-IP

    Plasmid
    #63001
    Purpose
    Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog N145A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Asparagine 145 (146 AA in Mouse Protein) …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63001-plasmid-map-sequence-id-104605

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  40. pPyCAG-Nanog(R147A)-IP

    Plasmid
    #63002
    Purpose
    Expresses Mouse Nanog R147A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog R147A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Arginine 147 (148 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63002-plasmid-map-sequence-id-104608

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