We narrowed to 9,334 results for: bli

1. pAAVS hOPM

   Plasmid

   #112498

   Purpose

   Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.

   Depositor

   Bruce Conklin

   Insert

   OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HaloTag-G protein gamma-5

   Plasmid

   #67039

   Purpose

   G protein gamma-5 tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-G protein gamma-5 (GNG5 Bovine)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. RPRD1B

   Plasmid

   #40739

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RPRD1B (RPRD1B Human)
   

   Tags

   6xHis TEV

   Expression

   Bacterial

   Available Since

   Nov. 14, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. mmu-miR-30a-5p Block-iT design (BLK)

   Plasmid

   #107789

   Purpose

   miR-30a RNAi Expression

   Depositor

   Fabio Penna

   Insert

   Mmu-miR-30a-5p mature sequence (Mir30a Mouse)
   

   Use

   RNAi

   Tags

   EmGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. DVL2

   Plasmid

   #38876

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DVL2 (DVL2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. PANK1

   Plasmid

   #32871

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PANK1 (PANK1 Human)
   

   Tags

   His tag (with Thrombin cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 231-597 only

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pLV-Enh eGFP Reporter-huKC2-IGR21

   Plasmid

   #59456

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   huKrt1 3’intergenic region
   

   Use

   Lentiviral

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-T7-ABEmax-SpG

   Plasmid

   #195278

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

   Depositor

   Carsten Lederer, Petros Patsali

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUltra-hot-H2B-mTagBFP2

   Plasmid

   #161792

   Purpose

   3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Insert

   H2B-mTagBFP2
   

   Use

   Lentiviral; Multicistronic

   Tags

   mCherry and mTagBFP2

   Expression

   Mammalian

   Mutation

   I174A in the mTagBFP2 protein according to PLoS O…

   Promoter

   UbC

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL2/GZ

    Plasmid

    #1282

    Depositor

    Susan Lindquist

    Article

    Kimura et al Science 1995 Jun 2;268(5215):1362-5.

    Insert

    GRE-lacZ
    

    Tags

    glucocorticoid response elemen-lacZ

    Expression

    Yeast

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pUASp YFP Ras64B WT

    Plasmid

    #52245

    Purpose

    Drosophila expression of YFP-Ras64B

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. p2Bac.HA.p52

    Plasmid

    #39219

    Depositor

    Aziz Sancar

    Insert

    p52 (GTF2H4 Human)
    

    Tags

    HA

    Expression

    Insect

    Promoter

    Pp10

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLV Agpat2-V5

    Plasmid

    #175159

    Purpose

    Lentiviral expression of mouse Agpat2-V5

    Depositor

    Larry Gerace

    Insert

    Agpat2 (Agpat2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SMYD3

    Plasmid

    #32048

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SMYD3 (SMYD3 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

    Plasmid

    #61847

    Purpose

    mammalian expression of CDK1 WT, used for Ips

    Depositor

    Jonathon Pines

    Insert

    CDK1 (CDK1 Human)
    

    Tags

    SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pKG187

    Plasmid

    #63077

    Purpose

    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXC9 (murine) HIS-tag pET

    Plasmid

    #8559

    Depositor

    Corey Largman

    Insert

    HOXC9 (Hoxc9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid

    #61850

    Purpose

    cell cycle marker

    Depositor

    Jonathon Pines

    Insert

    PCNA (PCNA Human)
    

    Tags

    NLS and mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

19. LGshp53CreER (self deleting)

    Plasmid

    #33341

    Depositor

    Scott Lowe

    Article

    Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

    Insert

    CreERT2
    

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpCas9_mouse_Adar1_ccB

    Plasmid

    #158122

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 4MTL

    Plasmid

    #60100

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL21C:PBC002-E06:C229066 (METTL21C Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pGEX-6P-Cdh1wt (570)

    Plasmid

    #39877

    Depositor

    Jonathon Pines

    Insert

    FZR1 (FZR1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pENTRY4_HuB

    Plasmid

    #65752

    Purpose

    entry vector with HuB

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hSyn-Aion-ts-mScarlet-ER

    Plasmid

    #192577

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-mScarlet-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLV Cnep1r1-V5

    Plasmid

    #175133

    Purpose

    Lentiviral expression of mouse Cnep1r1-V5

    Depositor

    Larry Gerace

    Insert

    Cnep1r1 (Cnep1r1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 4P7A

    Plasmid

    #61954

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pHyg-PCUP1-AID*(N)

    Plasmid

    #99529

    Purpose

    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Insert

    IAA17(71-117) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLEX307-CTSL-G418

    Plasmid

    #158468

    Purpose

    Constitutive lentiviral expression of human CTSL.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSL (CTSL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ENO3

    Plasmid

    #39072

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ENO3 (ENO3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. isoform PKC Zeta 2 (JCE863)

    Plasmid

    #182028

    Purpose

    yeast expression of mouse PKC Zeta isoform 2 with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Insert

    PKC Zeta isoform 2
    

    Expression

    Mammalian

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

    Plasmid

    #44081

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Available Since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTre-Tight-luciferase-Hottip (522)

    Plasmid

    #65490

    Purpose

    positive control for lncRNA expression assay

    Depositor

    Rory Johnson, Roderic Guigo

    Insert

    Hottip (HOTTIP Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSpot-Tag-Lamin

    Plasmid

    #117062

    Purpose

    For mammalian expression of lamin B1 with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    Lamin B1 (LMNB1 Human)
    

    Tags

    Spot-Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTRY4_HuD

    Plasmid

    #65754

    Purpose

    entry vector with HuD

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Expression

    Mammalian

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid

    #104419

    Purpose

    transient expression of dCas9-dp300 fusion protein

    Depositor

    Stephan Beck

    Insert

    dp300
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    S1396R-S1397R

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIC84

    Plasmid

    #63062

    Purpose

    Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    hCENP-H, IMAGE clone 3451767 (CENPH Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBACgus4x/Parkin

    Plasmid

    #40385

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Parkin (PRKN Human)
    

    Use

    Baculovirus

    Mutation

    WT

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

    Plasmid

    #44093

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKG402

    Plasmid

    #69760

    Purpose

    Retroviral expression of CENP-T aa 256-561

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 256-561 (CENPT Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pAAV asyn S129E

    Plasmid

    #36068

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S129E (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S129E

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only