4,181 results

1. pHAGE-KRAS-G12D

   Plasmid

   #116423

   Purpose

   Lentiviral expression of KRAS G12D

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   G12D

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A-1x2

   Plasmid

   #58766

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCardinal-N1

   Plasmid

   #54590

   Purpose

   Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mCardinal

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCMV-HA-UTX

   Plasmid

   #24168

   Purpose

   Mammalian expression of human UTX with HA tag

   Depositor

   Kristian Helin

   Article

   Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

   Insert

   UTX (KDM6A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LSL-Cas9-Rosa26TV

   Plasmid

   #61408

   Purpose

   Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   3xFLAG and P2A

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CAG

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PPRE-pNL1.3

   Plasmid

   #84394

   Purpose

   Secreted Luciferase reporter for PPARgamma activity

   Depositor

   Thierry Fournier, Severine Degrelle

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Tags

   secNluc

   Expression

   Mammalian

   Promoter

   PPRE

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7

   Plasmid

   #11795

   Purpose

   3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

   Depositor

   Luk Parijs

   Article

   Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV/TO SV40 small + Large T (w612-1)

   Plasmid

   #22298

   Depositor

   Eric Campeau

   Insert

   SV40 small + Large T antigen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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