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Showing: 2481 - 2500 of 4087 results

  2. pCDF-casBCDE(+6)

    Plasmid
    #89728
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a longer versi…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89728-plasmid-map-sequence-id-177602

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  4. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

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  5. TFORF3064

    Plasmid
    #144540
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TERF1 (TERF1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144540-plasmid-map-sequence-id-404245

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  6. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  7. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  8. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  15. pCDF-casBCDE(-6)

    Plasmid
    #89730
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89730-plasmid-map-sequence-id-177596

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  16. pCDF-casBCDE(-12)

    Plasmid
    #89731
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89731-plasmid-map-sequence-id-182925

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  17. pCDF-casBCDE(-18)

    Plasmid
    #89732
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89732-plasmid-map-sequence-id-177593

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  18. pCDF-casBCDE(-3)

    Plasmid
    #89729
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89729-plasmid-map-sequence-id-177590

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  19. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid
    #65454
    Purpose
    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    EYFP
    Expression
    Mutation
    H134R variant
    Promoter
    hSyn1
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65454-plasmid-map-sequence-id-436383

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Showing: 2481 - 2500 of 4087 results