We narrowed to 59,329 results for: cloning

1. Pf-KsgA

   Plasmid

   #25585

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Pf-KsgA
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Oct. 25, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. PP-PCMT

   Plasmid

   #25106

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PF14_0309:C34112
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. PP-FTSJ

   Plasmid

   #25107

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PF13_0052:C34076
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. PP-PEBP (2R77)

   Plasmid

   #25116

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PFL0955c:C36672
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. PP-PC5R

   Plasmid

   #25117

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MAL13P1.284:C34046
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pTcTurboID-GW_GFP

   Plasmid

   #234715

   Purpose

   Used to express GFP fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling cytoplasmatic spatial control. CDS can be cloned in frame to GFP using EcoRV.

   Depositor

   Esteban Serra

   Insert

   GFP
   

   Use

   Trypanosomatid expression

   Tags

   3xHA

   Promoter

   T7

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG Retro Neo Empty

   Plasmid

   #234460

   Purpose

   Retroviral vector for cloning and expressing your gene of interest. Neomycin selection.

   Depositor

   Addgene Research Program, Melina Fan

   Type

   Empty backbone

   Use

   Retroviral

   Available Since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAG Retro Hygro Empty

   Plasmid

   #234459

   Purpose

   Retroviral vector for cloning and expressing your gene of interest. Hygromycin selection.

   Depositor

   Addgene Research Program, Melina Fan

   Type

   Empty backbone

   Use

   Retroviral

   Available Since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pTD56

   Plasmid

   #172895

   Purpose

   mKate2^SEC^AID* vector with ccdB markers for cloning homology arms

   Depositor

   David Reiner

   Insert

   mKate2
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTD72

    Plasmid

    #172896

    Purpose

    mKate2^SEC^2xHA vector with ccdB markers for cloning homology arms

    Depositor

    David Reiner

    Insert

    mKate2
    

    Use

    CRISPR

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEos4b-Patched1-N-18

    Plasmid

    #62778

    Purpose

    Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Insert

    Patched1
    

    Tags

    mEos4b

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. Pb-OMPDC

    Plasmid

    #25573

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pb-OMPDC
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. Pv-ASL

    Plasmid

    #25587

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pv-ASL
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. Pv-UBC

    Plasmid

    #25592

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pv-UBC
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. TDRD2

    Plasmid

    #25210

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TDRD2_11 (TDRKH Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. PP-mACP

    Plasmid

    #25124

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    59.m03510:C40207
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pXTK024 [Origin_RSF1010_PartType1]

    Plasmid

    #229261

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    RSF1010 Origin
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCm C-term MBP NikJC199U

    Plasmid

    #174365

    Purpose

    Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Tags

    8xHis, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEmpty_Control

    Plasmid

    #162607

    Purpose

    Empty control for Cas9 experiments and to clone new guide RNAs for paper

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Expression

    Yeast

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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