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We narrowed to 25,138 results for: crispr

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Showing: 2481 - 2500 of 25138 results

  2. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid
    #155050
    Purpose
    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    Use
    CRISPR
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155050-plasmid-map-sequence-id-315172

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  3. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid
    #155049
    Purpose
    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    Use
    CRISPR
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155049-plasmid-map-sequence-id-308669

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  6. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid
    #200904
    Purpose
    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.
    Depositor
    Samuel H. Sternberg
    Publication
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    Expression
    Mammalian
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200904-plasmid-map-sequence-id-393155

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  7. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  11. pCRISPRi

    Plasmid
    #199808
    Purpose
    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus
    Depositor
    Steve Salipante
    Publication
    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA with GFP cassette
    Use
    CRISPR
    Promoter
    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    199808-plasmid-map-sequence-id-403016

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  18. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  19. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid
    #228724
    Purpose
    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228724-plasmid-map-sequence-id-454004

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Showing: 2481 - 2500 of 25138 results