We narrowed to 17,100 results for: emb

1. pPBpuro-EGFP-eDHFR(69K6)

   Plasmid

   #178850

   Purpose

   Mammalian expression of EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV113

   Plasmid

   #63180

   Purpose

   pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Akt2(K14A/R25E)

   Plasmid

   #86595

   Purpose

   Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(K14A/R25E) (AKT2 )
   

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCer-Vamp2(qv/fw)

   Plasmid

   #53238

   Purpose

   Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Mutated Q76 to V; F77 to W.

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pQCi-hTRAC-sgRNA

   Plasmid

   #154093

   Purpose

   pQCi backbone with sgRNA targeting human TCR-alpha common chain

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting human TRAC locus
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK563

   Plasmid

   #110612

   Purpose

   BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Nanoluc
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMM2-rPMCA2a

   Plasmid

   #47762

   Purpose

   mammalian expression of rat PMCA2a

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6

   Insert

   PMCA2a (Atp2b2 Rat)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBTK124

   Plasmid

   #110583

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP25 promoter with lac operator and RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBTK403

   Plasmid

   #110599

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pX458_miR-290-295KO-gRNA1

    Plasmid

    #172709

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458_miR-290-295KO-gRNA2

    Plasmid

    #172710

    Purpose

    Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV114

    Plasmid

    #63181

    Purpose

    pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPS0253

    Plasmid

    #115505

    Purpose

    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK401

    Plasmid

    #110597

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCIPpay-BFP reporter

    Plasmid

    #154096

    Purpose

    Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    P2A-tagBFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMM2-hPMCA4a

    Plasmid

    #47761

    Purpose

    mammalian expression of human PMCA4a

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Enyedi et al J Biol Chem. 1994 Jan 7;269(1):41-3.

    Insert

    PMCA4a (ATP2B4 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK130

    Plasmid

    #110584

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBTK550d

    Plasmid

    #110617

    Purpose

    BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pKT3Ts-goldyTALEN

    Plasmid

    #80330

    Purpose

    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

    Depositor

    Jeffrey Essner

    Article

    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Bacterial

    Promoter

    T3

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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