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pHRSin_1G4 hTCRβ-SNAP

Plasmid

#187573

Purpose

Lentiviral expression of hTCR beta(1G4), and SNAP

Depositor

Simon Davis

Publication

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 hTCR beta, and SNAP tag (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3C2G

Plasmid

#171946

Purpose

Expresses PIK3C2G in mammalian cells

Depositor

Cheng-han Yu

Publication

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

PIK3C2G (PIK3C2G Human)

Tags

EGFP

Expression

Mammalian

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAd5-B6-deltaE3

Plasmid

#122559

Purpose

Modified block 6, with deletion in E3

Depositor

Fred Bunz

Publication

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 27859-30803

Use

Synthetic Biology

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-3GFP-kanMX6

Plasmid

#105148

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3GFP-kanMX6

Tags

3GFP

Expression

Bacterial and Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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dsRedm-Rab21

Plasmid

#83425

Purpose

Full-length mouse Rab21 (wildtype)

Depositor

Johanna Ivaska

Publication

Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

Insert

Rab21 (Rab21 Mouse)

Tags

dsRed-monomer

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TAL2288

Plasmid

#36723

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCG-TALEN_Left (Fancg Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2255

Plasmid

#36698

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCC-TALEN_Right (Fancc Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
TAL2274

Plasmid

#36711

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Left (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-RbE2Fmut

Plasmid

#32391

Depositor

Julien Sage

Publication

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

Rb promoter (Rb1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated E2F binding site: TTTCCCGC --> TTTACATC

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-nMyc-wt_3'UTR

Plasmid

#31858

Depositor

Robert Blelloch

Publication

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

Plasmid

#218666

Purpose

sgRNA targeting human EIF2AK3/PERK

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Publication

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19/mChe-PM

Plasmid

#183168

Purpose

Construct for transient expression of plasma membrane-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Publication

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-PM

Tags

OsRac3, O. sativa rac-like GTP-binding protein 3

Expression

Plant

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-mPCSK8

Plasmid

#122677

Purpose

Expresses mouse PCSK8 in mammalian cells

Depositor

Liming Pei

Publication

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )

Expression

Mammalian

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

Plasmid

#109311

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

Depositor

William Lagor

Publication

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Insert

Ldlr gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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Venus rGBD

Plasmid

#99015

Purpose

Used as a RhoA-GTP sensor

Depositor

Narasimhan Gautam

Publication

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

rGBD (Rtkn Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:S10-(MCS)-3xHA

Plasmid

#108178

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Publication

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FCIV1-GR

Plasmid

#72701

Purpose

Lentiviral expression of rat GR (2nd generation)

Depositor

Freddy Jeanneteau

Publication

Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Promoter

ubiquitin

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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