We narrowed to 6,950 results for: ust

1. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   601 has an additional mutation added 22 bp from d…

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-ScChd1[R126A/R130A]

   Plasmid

   #221197

   Purpose

   ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   R126A, R130A

   Promoter

   T7

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST17-ScChd1[∆ChEx]

   Plasmid

   #221199

   Purpose

   ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa142-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-117 and 1275-1468

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET SUMO-syt1 (96-421)

   Plasmid

   #213619

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 (96-421)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 (96-421) (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET SUMO-syt1 (96-421) I367C

   Plasmid

   #213620

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 (96-421) I367C (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   C277A; I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET SUMO-syt7 (136-403)

   Plasmid

   #213621

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 (136-403)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 (136-403) (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET SUMO-syt1 5W

   Plasmid

   #213623

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET SUMO-syt1 s7+

   Plasmid

   #213624

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 s7+

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 s7+ (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET SUMO-syt7 C2AB - 4A

   Plasmid

   #213625

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB - 4A (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   F167A, F229A, I298A, L361A

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET SUMO-syt7 C2A-2A 2B

    Plasmid

    #213626

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2A-2A 2B (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    F167A, F229A, R363H

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET SUMO-syt7 C2AB-2A

    Plasmid

    #213627

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB-2A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    I298, L361A

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET SUMO-FL-syt1 5W

    Plasmid

    #213629

    Purpose

    Bacterial expression of SUMO-tagged rat FL-syt1 5W

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt1 5W (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    M173W, F231W, F234W, C277A, V304W, Y364W, I367C

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYN2_1

    Plasmid

    #184757

    Purpose

    Plasmid for genome editing by CRISPR/Cas9

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    

    Use

    Synthetic Biology

    Tags

    2xNLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    PGK1 and tRNA-Phe-HDV Ribozyme

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. RGG-BcLOV-mCh-EGFR

    Plasmid

    #208280

    Purpose

    Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    RGG-BcLOV-mCh-EGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    H225N in mCherry- please see depositor comment

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Combined γB2FL-FRET probe

    Plasmid

    #209094

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Combined γB2ΔICD-FRET probe

    Plasmid

    #209093

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hsyn-dMASS_1_WPRE

    Plasmid

    #209765

    Purpose

    AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-2T_RV-B14_VP1

    Plasmid

    #202074

    Purpose

    Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    RV-B14 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Shim_CytC_TP_GFP_pK7FWG2

    Plasmid

    #202646

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative Cytochrome C
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGEX-2T_RV-A89_VP1

    Plasmid

    #202073

    Purpose

    Bacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    RV-A89 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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