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pFM041
Plasmid#40144DepositorInsertpar-2 RNAi resistant (S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationS241A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM039
Plasmid#40142DepositorInsertpar-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + S241A; base pairs 6-523 of par-2 were …Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM036
Plasmid#40139DepositorInsertpar-2 RNAi resistant (K162A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationK162A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pRLCon1288-1666
Plasmid#31448DepositorInsertHNF4A 3'UTR fragment (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221_850-1207
Plasmid#31449DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221mut2_850-1207
Plasmid#31450DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationmut2 in balancerPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon1-449_850-1207
Plasmid#31451DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-hARdelta120-472
Plasmid#89088Purposemammalian expression of human androgen receptor with deletion of 120-472 amino acid residuesDepositorInserthAR-120-472 N-term deletion (AR Human)
UseTagsExpressionMammalianMutationhAR-120-472PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p026 pCS2-EnR
Plasmid#11028DepositorInsertengrailed (en Fly)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationEngrailed repressor domain (bases encoding amino …PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-H (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVTagsExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-M
Plasmid#182284PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
polyQ74-GFP
Plasmid#231893PurposeForms cytosolic HTT partial exon 1 Q74 aggregatesDepositorInsertHTT partial exon 1 Q74 (HTT Human)
UseTagsEGFPExpressionMammalianMutationCAG repeat expansionPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi1-LgB91
Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorInsertGαi1-LgB91 (GNAI1 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL119-TK
Plasmid#21911DepositorInsertHSV Type 1 thymidine kinase
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UUA)_EF1_MmaPylRS
Plasmid#174896PurposeMma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsExpressionMammalianMutationPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1364 LV EF1a-CD4 IRES-EGFP
Plasmid#201920Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD4DepositorInsertCD4 (CD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorInsertLAMP1-TORCAR (LAMP1 Synthetic, Human)
UseTagsCerulean, Full-length LAMP1, and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UCA)_EF1_MmaPylRS
Plasmid#174899PurposeMma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
MSCV-XZ066-EGFRvIII
Plasmid#20737DepositorInsertEGFRvIII (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationEGFR with a 267 amino acid deletion in the extrac…PromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only
