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pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

Plasmid

#188767

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-BRD4ΔN-mCherry-sspB

Plasmid

#121968

Purpose

Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACT2-CAS9c

Plasmid

#51055

Purpose

Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

Cas9c

Use

CRISPR

Tags

SV40 NLS

Expression

Bacterial and Yeast

Promoter

yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pmCTIP2-N174

Plasmid

#60858

Purpose

Expresses CTIP2 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Publication

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pEG BacMam C term 3C eGFP His8

Plasmid

#160687

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP His8

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-WT

Plasmid

#194582

Purpose

Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

Depositor

Denes Hnisz

Publication

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SQSTM1 (SQSTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Publication

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-NKX6.1

Plasmid

#32934

Depositor

Kevin Eggan

Publication

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

NKX6.1 (NKX6-1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPar2

Plasmid

#53228

Purpose

mammalian expression of human Par2

Depositor

Shaun Coughlin

Publication

Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Par2 (F2RL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Publication

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Publication

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCry2PHR-mCh-LRP6c

Plasmid

#42960

Purpose

Expresses optogenetically controlled LRP6 (C-terminal domain)

Depositor

David Schaffer

Publication

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cry2PHR-mCherry-LRP6c

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

CMV

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Publication

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRcCMV myc-Plk1 T210D (Nigg LA183)

Plasmid

#41158

Depositor

Erich Nigg

Publication

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

T210D

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Publication

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Publication

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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