We narrowed to 3,184 results for: actin

1. Coexpressed VLP NES (G)

   Plasmid

   #182477

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(G)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, GAL10, and GAL7

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Linked Free NES (AG4TGGA)2

   Plasmid

   #182488

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Linked Free (PT)4P

   Plasmid

   #182490

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDTCendoNA2

   Plasmid

   #174732

   Purpose

   Expresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linker

   Depositor

   Jukka Finne

   Article

   Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

   Insert

   DT386C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Deleted amino acids 412-560 (the receptor binding…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

   Plasmid

   #166855

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K683E, H685E, K686E

   Promoter

   pCu

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

   Plasmid

   #166854

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641E, I645E

   Promoter

   pCu

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

   Plasmid

   #166853

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641G, I645G

   Promoter

   pCu

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ISDra2-TnpB-mCherry-U6-ωRNA*

   Plasmid

   #212967

   Purpose

   Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

   Depositor

   Chunlong Xu

   Article

   Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

   Inserts

   ISDra2-TnpB-T2A-mCherry
   ωRNA*
   

   Use

   CRISPR

   Tags

   NLS and NLS-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, T7 and U6

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

   Plasmid

   #228902

   Purpose

   For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

   Depositor

   Bryan Roth

   Article

   Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

   Insert

   hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
   

   Use

   AAV and Synthetic Biology

   Tags

   HA and P2A mCitrine

   Expression

   Mammalian

   Mutation

   R108K

   Promoter

   Chicken Beta Actin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS SARS-CoV-2 D614G Spike

    Plasmid

    #185692

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 D614G Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 D614G Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutation: D614G

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCaggs-mGold2t

    Plasmid

    #231764

    Purpose

    Expression of mGold2t in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid

    #206115

    Purpose

    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV/B-actin

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL Neo Myr Flag TWF2

    Plasmid

    #20602

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    TWF2 (TWF2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pGBT-RP8.1

    Plasmid

    #189846

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid

    #206098

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A, E664A, E1370A, E1658A

    Promoter

    CMV/B-actin

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBT-RP2.2

    Plasmid

    #189842

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBT-RP2.3

    Plasmid

    #189843

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBT-RP8.3

    Plasmid

    #189848

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-ataxin-3 *VBM

    Plasmid

    #89976

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 282RKRR to 282HNHH

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIV-EGFP.Flag-EZH2-mt1

    Plasmid

    #220244

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R27A, R31A, F165A

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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