We narrowed to 8,683 results for: sgRNA

1. pLenti CRISPR sgSHMT2_2

   Plasmid

   #106310

   Purpose

   Express Cas9 and sgRNA targeting SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. APq5210

   Plasmid

   #66086

   Purpose

   co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

   Insert

   sgRNA for APa4-2
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. MIGR1-U6gRNA2-Filler

   Plasmid

   #237401

   Purpose

   For the sequential insertion of two guide RNAs into two U6gRNA cassettes.

   Depositor

   Zuoming Sun

   Article

   Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

   Inserts

   U6gRNA-1
   U6gRNA-2
   PGK-TagBFP
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   PGK promoter, U6 promoter, and U6, PGK

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Cas9-NS-A

   Plasmid

   #207823

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Cas9-NS-B

   Plasmid

   #207824

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA_hMECP2_promoter_No8

   Plasmid

   #194902

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.8
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgRNA_hMECP2_promoter_No6

   Plasmid

   #194900

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.6
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgRNA_hMECP2_promoter_No7

   Plasmid

   #194901

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.7
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLiP-G2

   Plasmid

   #188964

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PrhaBAD

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPQDKN0760

    Plasmid

    #185629

    Purpose

    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD0KN0751

    Plasmid

    #185628

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW-shRIIα

    Plasmid

    #183454

    Purpose

    sgRNA targeting rat PKA-RIIα subunits

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
    

    Use

    Lentiviral

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDIV495

    Plasmid

    #177702

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT-9251

    Plasmid

    #124226

    Purpose

    Bacterial SpCas9-HF1 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)-Cdt1

    Plasmid

    #122342

    Purpose

    Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Cdt1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ141-ZmUbi-RZ-Aa1.2

    Plasmid

    #136373

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CRISPR V2 sgMTHFD1L_2

    Plasmid

    #106317

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP303-4

    Plasmid

    #66088

    Purpose

    co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs1
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only