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pGAL4-DBD-EGR1-AroLITE_A

Plasmid

#215593

Purpose

For overexpression of Gal4-DBD EGR1 AroLITE A

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroLITE A (EGR1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HOXD4_AroPLUS

Plasmid

#215628

Purpose

For integration of HOXD4 AroPLUS mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS (HOXD4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS_LITE

Plasmid

#215583

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS LITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS LITE (HOXD4 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroPATCHY1

Plasmid

#215596

Purpose

For overexpression of Gal4-DBD EGR1 AroPATCHY1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroPATCHY1 (EGR1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroPERFECT_IS10

Plasmid

#215606

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS10

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS10 (CEBPA Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroLITE_A

Plasmid

#215602

Purpose

For overexpression of Gal4-DBD CEBPa AroLITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroLITE (CEBPA Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209061

Purpose

Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

KDM6A

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q)

Plasmid

#200979

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q mutation fo…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EFNB2 (D62Q)

Plasmid

#200976

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q (increases specificity towards henipaviral G…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lamp1 FLAG

Plasmid

#199750

Purpose

Lentiviral construct to stably express Lamp1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Lamp1 (Lamp1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc Metap2

Plasmid

#199685

Purpose

Transient expression of Metap2

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-M135R-EGFP

Plasmid

#164944

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135R

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted; M135R

Promoter

actinb (actb2) promoter

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00004

Plasmid

#166721

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

Plasmid

#160560

Purpose

tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

M3_3-pTRNA-scf 2.1

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

Plasmid

#160558

Purpose

tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

M1_3-pTRNA-scf 2.1

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

Plasmid

#164945

Purpose

Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

KCNJ13 (KCNJ13 Human)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-Q153H-EGFP

Plasmid

#164943

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted; Q153H; M154I

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-T131A-EGFP

Plasmid

#164942

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted; T131A; G133R

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

Plasmid

#160559

Purpose

tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

M2_3-pTRNA-scf 2.1

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA sCRNA 2.0 (GB2461)

Plasmid

#160593

Purpose

Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA sCRNA 2.0

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

Plasmid

#160625

Purpose

GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

U6-26-1gRNA-DFR 2.1

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (PPP)

Plasmid

#62067

Purpose

Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A, P27A

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pCDH-EWSR1::WT1(-KTS)

Plasmid

#220565

Purpose

Lentiviral expression of human EWSR1::WT1(-KTS)

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

EWSR1::WT1(-KTS) (EWSR1, WT1 Human)

Use

Lentiviral

Tags

HA

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EWSR1::WT1(+KTS)

Plasmid

#220566

Purpose

Lentiviral expression of human EWSR1::WT1(+KTS)

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

EWSR1::WT1(+KTS) (WT1, EWSR1 Human)

Use

Lentiviral

Tags

HA

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-eGFP-Puro

Plasmid

#79823

Purpose

Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly

Plasmid

#70765

Purpose

Expresses mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Cre targeting vector

Plasmid

#61570

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb exon 4 - 2A - Cre

Use

Cre/Lox and Mouse Targeting

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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