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Addgene

We narrowed to 10,599 results for: cat.1

Showing: 5081 - 5120 of 10599 results
  1. p5E-mpeg1.1

    Plasmid
    #75023
    Purpose
    Can be used to drive expression in zebrafish microglia.
    Depositor
    Insert
    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Tags
    Expression
    Mutation
    Promoter
    mpeg1.1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173202
    Purpose
    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid
    #173200
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid
    Depositor
    Insert
    ATP1A1 G6 T804N pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pBItetDT12nGFP

    Plasmid
    #80420
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located
    Depositor
    Insert
    EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pX330_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173205
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. FLuc-N-HiBiT

    Plasmid
    #207117
    Purpose
    Bacterially expressed HiBiT-FLuc for Ni-NTA purification
    Depositor
    Insert
    luciferin 4-monooxygenase
    Use
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. FLuc-C-HiBiT

    Plasmid
    #207118
    Purpose
    Bacterially expressed FLuc-HiBiT for Ni-NTA purification
    Depositor
    Insert
    luciferin 4-monooxygenase
    Use
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. FLuc-C-NLuc

    Plasmid
    #234566
    Purpose
    Bacterially expressed FLuc-C-NLuc for Ni-NTA purification
    Depositor
    Insert
    Firefly luciferase
    Use
    Tags
    NanoLuciferase-thrombin site-10xHis and T7 gene 1…
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid
    #173212
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.
    Depositor
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid
    #178090
    Purpose
    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.
    Depositor
    Insert
    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pBItetDT960GFP

    Plasmid
    #80419
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 located
    Depositor
    Insert
    EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 5081 - 5120 of 10599 results