We narrowed to 18,902 results for: Met;

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pSY14-ptb

Plasmid

#90490

Purpose

pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRATIO1112

Plasmid

#141066

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-IT5

Plasmid

#66869

Purpose

Inntag 5: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 5

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Clover-Lifeact-7

Plasmid

#54551

Purpose

Localization: Actin, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Clover-Lifeact

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
M-tdTom-NM

Plasmid

#48679

Purpose

Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
pGTag Hygro EGFP nLAP +0

Plasmid

#194305

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pReporter_2

Plasmid

#60563

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_2

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pcDNA3

Plasmid

#67771

Purpose

Kohinoor for mammalian cell expression in cytosol

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pBPHLWL-GAL4DBD

Plasmid

#26256

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

ZIP- Gal4DBD (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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PH-dL5**

Plasmid

#96946

Purpose

PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

dL5 and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Talin-N-10

Plasmid

#55139

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

Insert

Talin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pRATIO2212

Plasmid

#141302

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-PH-dL5**

Plasmid

#96947

Purpose

Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

EGFP and dL5

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Actin/pcDNA3

Plasmid

#67776

Purpose

Actin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pXL-BACII-attPGAL4LWL

Plasmid

#26260

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pAG25-L-IFP-F[1] (clon 3)

Plasmid

#52899

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Vector for homologous recombination

Promoter

TEF promoter

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only