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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 75,816 results for: Rest

Showing: 2541 - 2560 of 75816 results

  1. pCJR01

    Plasmid
    #187888
    Purpose
    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Insert
    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187888-plasmid-map-sequence-id-368412

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  3. psiCHECK2-let-7 WT

    Plasmid
    #78260
    Purpose
    psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    fully complimentary let-7 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78260-plasmid-map-sequence-id-149453

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  4. Barcode backbone

    Plasmid
    #158665
    Purpose
    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    hcRED
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158665-plasmid-map-sequence-id-309764

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  10. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  12. pLenti-TetO-PM

    Plasmid
    #197387
    Purpose
    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
    Depositor
    Qiao Zhou
    Article
    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038
    Insert
    PDX1-T2A-MAFA
    Use
    Lentiviral; Teto
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    197387-plasmid-map-sequence-id-385579

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  16. pLyGo-Ec-8

    Plasmid
    #163136
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P(rhaB)
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163136-plasmid-map-sequence-id-332138

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  18. pCLucIPZ

    Plasmid
    #53222
    Purpose
    Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest
    Depositor
    Thorsten Stiewe
    Article
    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1
    Inserts
    Cypridina luciferase
    non-silencing shRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53222-plasmid-map-sequence-id-342548

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  19. pCAP01

    Plasmid
    #59981
    Purpose
    The function of this material is to facilitate the capture and expression of bacterial gene clusters.
    Depositor
    Bradley Moore
    Article
    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111
    Type
    Empty backbone
    Use
    Synthetic Biology; Transformation-associated reco…
    Tags
    no
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    no
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59981-plasmid-map-sequence-id-96247

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Showing: 2541 - 2560 of 75816 results