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PiggyBac-FLAG-hTET2-IRES-Blast

Plasmid

#239819

Purpose

PiggyBac vector to express full length human TET2 with 2X N-terminal FLAG tags

Depositor

Kasper Rasmussen

Publication

Knatko et al Blood Adv. 2025 Jun 24:bloodadvances.202

Insert

FLAG-tagged human TET2 (TET2 Human)

Tags

2x FLAG tag on TET2

Expression

Mammalian

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hMLKL

Plasmid

#208789

Purpose

Inducibly expresses human MLKL, fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MLKL (MLKL Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-FLAG-NEK9

Plasmid

#168269

Purpose

Expresses NEK9 in mammalian cells

Depositor

Noboru Mizushima

Publication

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pmMaple3-CAM

Plasmid

#101148

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Publication

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple3

Expression

Bacterial

Promoter

pBAD

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pETM6-mCherry

Plasmid

#66534

Purpose

Expresses mCherry under the control of the Concensus T7 Promoter

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mTagRFP-T-LaminB1-10

Plasmid

#58020

Purpose

Localization: Nuclear Envelope, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LaminB1 (LMNB1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Publication

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pCLUC

Plasmid

#1280

Depositor

Susan Lindquist

Publication

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

CUP1-lacZ fusion (CUP1-1 Budding Yeast)

Tags

CUP1 promoter

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pKT0101

Plasmid

#8721

Depositor

Kurt Thorn

Publication

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0212

Plasmid

#8725

Depositor

Kurt Thorn

Publication

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Tom7

Plasmid

#66571

Purpose

Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

Depositor

Orion Weiner

Publication

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

PhyB (PHYB Mustard Weed)

Tags

Tom7 and mCherry

Expression

Yeast

Mutation

PhyB portion contains aa's 1-908

Promoter

pADH1

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Publication

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CMV10-Flag-mMcl-1 KR

Plasmid

#32979

Depositor

Joseph Opferman

Publication

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Tags

3XFlag

Expression

Mammalian

Mutation

All lys changed to Arg

Promoter

CMV

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-PERK(LD)-EGFP

Plasmid

#225680

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

Depositor

Likun Wang

Publication

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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CMPE-ePPEplus

Plasmid

#205242

Purpose

For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho*-BERKY3

Plasmid

#158220

Purpose

BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFRT/TO/HIS/FLAG/HA-KIAA1967

Plasmid

#38082

Depositor

Markus Landthaler

Publication

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

KIAA1967 (CCAR2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Publication

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
SI2-Unc5b

Plasmid

#28215

Depositor

Gavin Wright

Publication

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Unc5b mouse monoclonal antibody (unc5b Zebrafish)

Expression

Mammalian

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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