1,878 results

1. pGM32DEST

   Plasmid

   #74747

   Purpose

   Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

   Depositor

   Keith Yamamoto, Jordan Ward

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Inserts

   Gateway cassette
   QF-GR (NR3C1 Neurospora crassa, Human, Synthetic)
   

   Tags

   Fusion of the Neurospora crassa QF activator to t…

   Expression

   Worm

   Promoter

   No promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCG142

   Plasmid

   #17246

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   pie-1 intron:pie-1 promoter in PDONRP4P1R
   

   Use

   Gateway

   Available Since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pmec-18 cz::ced-3 (p17) [TU#807]

   Plasmid

   #16081

   Depositor

   Martin Chalfie

   Article

   Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

   Insert

   cz:: ced-3 (p17) (ced-3 Nematode)
   

   Expression

   Worm

   Available Since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNM3725

   Plasmid

   #154113

   Purpose

   UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ909

   Plasmid

   #44480

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW2172

   Plasmid

   #163096

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1586

   Plasmid

   #121057

   Purpose

   mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mKate2-T^SEC^AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, C. elegans codon optimized mKate2, and au…

   Expression

   Worm

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJS007

   Plasmid

   #188330

   Purpose

   PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::wrmScarlet::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM3 = ncs-1p::nCre

   Plasmid

   #24034

   Depositor

   Cori Bargmann

   Article

   Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

   Insert

   ncs-1 (ncs-1 Nematode)
   

   Use

   Cre/Lox

   Tags

   nCre

   Expression

   Worm

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJW1836

    Plasmid

    #154337

    Purpose

    Promoter reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
    

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBN154

    Plasmid

    #86718

    Purpose

    FLP/Frt dual color reporter for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
    

    Expression

    Worm

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF11-wmiRFP670-2-T2A-HO1

    Plasmid

    #197250

    Purpose

    Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP670-2-T2A-HO1
    

    Expression

    Worm

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ210 - pDESTR4-R3(ttTi4348, I)

    Plasmid

    #34863

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi4348 targeting
    

    Use

    Worm targeting

    Available Since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM1.128

    Plasmid

    #21385

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    spe-11 promoter (no ATG) in pDONRP4P1R
    

    Use

    Gateway

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGLOW39

    Plasmid

    #173068

    Purpose

    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    mScarlet-I-C1^SEC^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mScarlet

    Expression

    Worm

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOD2048-csnDEG

    Plasmid

    #89368

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Promoter

    Posm-6

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pSM08

    Plasmid

    #139042

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mscarlet (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMT3-FBF2

    Plasmid

    #166972

    Purpose

    Expresses FBF-2 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOL2339

    Plasmid

    #37839

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-V
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    nhx-2

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOL2340

    Plasmid

    #37841

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-M
    

    Tags

    mCherry

    Expression

    Worm

    Mutation

    R77M

    Promoter

    nhx-2

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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