2,223 results
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Plasmid#117772PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-QF2
Plasmid#122563PurposeIntegration of QF2 sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-QF2-SV40pA
UseCloningAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:ECFP
Plasmid#113880Purposep3E entry vector for Three Multisite GatewayDepositorAvailable SinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a
Plasmid#107593PurposeAn entry vector with U6a promoter driving guide RNA expressionDepositorInsertU6a promoter
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
ExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1:GFP
Plasmid#105646PurposeIkk1(alpha) expression in basal keratinocytesDepositorAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterflk1Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3b
Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-slc6a3[11kb]:egfp
Plasmid#53240Purposeallow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthoodDepositorInsertDAT-GFP
UseCloningTagsGFPAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
374 pME-ets1a
Plasmid#49000Purposefull length coding sequence for zebrafish ets1a gene in gateway middle entry vectorDepositorInsertv-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
UseGateway middle entryAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7mitfagRNA
Plasmid#47931Purposein vitro transcription of mitfa gRNADepositorInsertmitfa gRNA (mitfa Zebrafish)
UseCRISPRAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0001
Plasmid#42241PurposegRNA targeted to zebrafish gene apoeaDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Dusp6_2KBpromoter
Plasmid#32666DepositorAvailable SinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
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Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546)
Plasmid#28071DepositorInsertZinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
UseZebrafish targetingAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS ZfHoxb1a
Plasmid#27519DepositorInsertHoxb1a (hoxb1a Zebrafish)
UseRiboprobe synthesisAvailable SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only