2,162 results

1. pUAS-Ncad-GFP

   Plasmid

   #35986

   Depositor

   Stephen Smith, James Jontes

   Article

   Jontes et al J Neurosci. 2004 Oct 13;24(41):9027-34.

   Insert

   Ncad (cdh2 Zebrafish)
   

   Use

   Gal4 inducible

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSYC-437

   Plasmid

   #163829

   Purpose

   Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

   Depositor

   Seok-Yong Choi

   Article

   Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

   Inserts

   Foxj1a Promoter
   GCaMP6s
   

   Use

   Multisite gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-sod1

   Plasmid

   #107594

   Purpose

   Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   sod1 (sod1 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-Isce1-eno2:egfp

   Plasmid

   #61394

   Purpose

   Expresses GFP in neurons of transgenic zebrafish

   Depositor

   Edward Burton

   Article

   Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

   Insert

   eno2 regulatory element
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   eno2

   Available Since

   Dec. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPRISM-Stop-gcry-mRFP

   Plasmid

   #117772

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   3x3 Stop sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZE11 tiggywinkle hedgehog CMV

   Plasmid

   #17060

   Depositor

   Randall Moon

   Insert

   tiggywinkle hedgehog (shhb Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pPRISM-Cre-cmlc2-eGFP

   Plasmid

   #117794

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-zAsCpf1

   Plasmid

   #107185

   Purpose

   plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   AsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hox D4a

   Plasmid

   #36469

   Depositor

   Robert Ho

   Article

   Prince et al Development. 1998 Feb . 125(3):407-20.

   Insert

   Hox D4a (hoxd4a Zebrafish)
   

   Use

   Riboprobe synthesis

   Tags

   Expression

   Bacterial

   Mutation

   Q100H, A101T, G125C and R189W

   Promoter

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid

    #98096

    Purpose

    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio alpha A-crystallin 1 kb fragment (-1028/-1)

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK074 p5E exorh:EGFP

    Plasmid

    #195951

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TALEN1257

    Plasmid

    #32280

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting hey2 (hey2 Zebrafish)
    

    Use

    T7

    Tags

    3X FLAG and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGTag-eGFP-B-actin

    Plasmid

    #117814

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2_claudink:Cas9green; erbb2_gRNA171

    Plasmid

    #199339

    Purpose

    pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. zCREST3:Cas9green; EGF_gRNA

    Plasmid

    #199333

    Purpose

    pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 EGF sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2_claudink:Cas9green; control_gRNA

    Plasmid

    #199336

    Purpose

    pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+-Pard3-EGFP-PIF6

    Plasmid

    #154914

    Purpose

    Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).

    Depositor

    Clare Buckley, Jonathan Clarke

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    1-100 amino acids of Arabidopsis PIF6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGTag-eGFP-CAAX-SV40

    Plasmid

    #117815

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135766

    Purpose

    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR - CaSR-EGFP

    Plasmid

    #191503

    Purpose

    A zebrafish CaSR-EGFP construct used for Gateway cloning.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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