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Plasmid#43903DepositorInsertUSP44 (USP44 Human)
UseTagsHISExpressionMammalianMutationC282APromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2
Plasmid#112842Purposetransient/stable overexpression of the central domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationC1 domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM beta catenin (CT#593)
Plasmid#13938DepositorInsertbeta catenin (CTNNB1 Chicken)
UseTagsExpressionBacterialMutationAmino acids 64-272PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-GINKO2
Plasmid#234544Purposeto express a potassium sensor tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-GINKO2
UseAAVTagsExpressionMutationNonePromoterP3Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-mNeonGreen
Plasmid#234543Purposeto express a green fluorescent protein tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-mNeonGreen
UseAAVTagsExpressionMutationNonePromoterP3Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-SEP
Plasmid#234541Purposeto express a pH sensor tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-SEP
UseAAVTagsExpressionMutationNonePromoterP3Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut
Plasmid#235591PurposeDox-inducible expression of control catalytic-mutant Dot1l CD fused with scFVDepositorInsertDot1l (Dot1l Mouse)
UseCRISPRTagsExpressionMutationGS163-164RCPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut
Plasmid#235590PurposeDox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFVDepositorInsertKmt5c (Kmt5c Mouse)
UseCRISPRTagsExpressionMutationNHDC182-185AAAGPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Setd2CD-CatMut
Plasmid#235588PurposeDox-inducible expression of control catalytic-mutant Setd2 CD fused with scFVDepositorInsertSetd2 (Setd2 Mouse)
UseCRISPRTagsExpressionMutationR1599CPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut
Plasmid#235583PurposeDox-inducible expression of control catalytic-mutant Ring1b CD fused with scFVDepositorInsertRing1b (Rnf2 Mouse)
UseCRISPRTagsExpressionMutationI53SPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLIX403 RNaseH1 Catalytic Inactive
Plasmid#237796PurposeInducible lentiviral expression of RNaseH1 Catalytic InactiveDepositorInsertRNaseH1 (RNASEH1 Human)
UseTagsExpressionMammalianMutationD210NPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
cASIC1 concatemer - fully assembled
Plasmid#233133PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cellsDepositorInsertASIC1 (ASIC1 Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-SpyCatcher3
Plasmid#235149PurposeBacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertSpyCatcher3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 817 Truncation
Plasmid#234126PurposeSyf1 PCR truncation starting at 817 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 634 Truncation
Plasmid#234116PurposeSyf1 PCR truncation starting at 634 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 778 Truncation
Plasmid#234115PurposeSyf1 PCR truncation starting at 778 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK059 [Con-Pro_cat-Broad_PartType8]
Plasmid#229296PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_cat-Broad_PartType8DepositorInsertcat Promoter (Broad)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK040 [Con-Pro_cat-Broad_PartType5]
Plasmid#229277PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_cat-Broad_PartType5DepositorInsertcat Promoter (Broad)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
cASIC1 concatemer - vector backbone
Plasmid#233132PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-catΔ
Plasmid#222508PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterTRE3GV and hPGKAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLViN-iRFP670-α-cateninA+ΔβH
Plasmid#229707PurposeLentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459-CTNNA1(α-catenin CRISPR KO)
Plasmid#229708PurposeKnockout of a-catenin in mammalian cells using CRISPR-Cas9 technologyDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-beta catenin tag
Plasmid#186422PurposeEncodes GST fused to beta catenin tag recognized by anti-beta catenin nanobodyDepositorInsertGST-beta catenin tag
UseTags6His and GST-beta catenin tag (PDRKAAVSHWQQRS)ExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher (Min)
Plasmid#216713PurposeExpresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014DepositorInsertCys-SpyCatcher (Min)
UseTags6xHisExpressionBacterialMutationSee Li...Howarth, J. Mol. Biol., 2014PromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB-EXPR(PYL-cat_dom_HDAC5)
Plasmid#114401PurposeFor PYL-HDAC5 catalytic domain expressionDepositorInsertPYL-HDAC5 catalytic domain
UseTags3xFlag-NLS (internal)ExpressionMammalianMutationS220F and the deletion of M221PromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24-aCatenin(82-906)-His6
Plasmid#203332PurposeBacterial expression of wild-type alpha-cateninDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags6xHisExpressionBacterialMutationaa 82-906PromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKAcat(G1A)-mCherry
Plasmid#181852PurposemCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
UseTagsmCherryExpressionMammalianMutationGlycine residue immediately after start site in P…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-SpyCatcher
Plasmid#179677PurposeHCVP-cleavable ER-associated SpyCatcherDepositorInsertHCVP SpyCatcher
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
UseTagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-Zdk1-Vav2cat
Plasmid#178875PurposeMammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1DepositorInsertmCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti
Plasmid#138312PurposeLenti expression of AcrIIA14, C-terminusDepositorInsertAcrIIA14, C terminus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3
Plasmid#112843Purposetransient/stable overexpression of the C-terminal domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationC2 domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1
Plasmid#112841Purposetransient/stable overexpression of the N-terminal domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationN domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat- (E77Q;E78Q)
Plasmid#41886DepositorInsertKaiC CI cat -(E77Q;E78Q)
UseTagsExpressionBacterialMutationchanged glutamic acid 77 and 78 to glutaminePromotert7 promoterAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat- (E318Q)
Plasmid#41887DepositorInsertKai C CII cat (E318Q)
UseBl de3 21TagsExpressionBacterialMutationchanged amino acid 318 E to QPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE74 TM Beta-catenin 1-9 in CS2+
Plasmid#16832DepositorInsertTM beta-catenin 1-9 (ctnnb1.L Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVTagsExpressionMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
123_pAAV-ProC1-CatCh-GFP-WPRE
Plasmid#125937PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProC1Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only