We narrowed to 803 results for: tev cleavage
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Plasmid#119967PurposeBacterial expression for structure determinationDepositorInsertLOC149281 (METTL11B Human)
Tags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 58-278PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
4QL1
Plasmid#61942PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5:APC041-G05:C36415 (WDR5 Human)
Tags6x His and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22b-EC12-129/138Avi
Plasmid#47927PurposeExpresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi tag in E.Coli.DepositorInsertE-Cadherin EC12
Tags6xHis, Avi Tag, and TEV CleavageExpressionBacterialMutationMutations C9A, K129C, D138CPromoterT7Available SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
GMIP
Plasmid#32046PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGMIP (GMIP Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 80-357 onlyAvailable SinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRE2-Bla(HA–RILP)
Plasmid#102425PurposeHA tag fused to the N-terminus of RILP for the expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
TagsHAExpressionMammalianPromoterTet-responsiveAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Bla(HA–RILP-FLAG)
Plasmid#102424PurposeDual tagged RILP for expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
TagsFLAG and HAExpressionMammalianPromoterTet-ResponsiveAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCsy_complex
Plasmid#89232PurposePlasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage siteDepositorInsertsCsy1
Csy2
Csy3
Csy4
Tags6xHis-TEV proteaseAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-H-Lock
Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pOPTM p62 PB1 ZZ (1-167)
Plasmid#210429PurposeBacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage siteDepositorAvailable SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)Available SinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
CLAPon
Plasmid#213405PurposePeroxidase (APEX) reporter for TEV protease cleavageDepositorInsertE5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0281
Plasmid#162302PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, [S-tag]-[TEV cleavage site]
UseSynthetic BiologyAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGC4780
Plasmid#113408PurposeTranslation reporter with inducible translation coupling (high native GFP expression)DepositorInsert158_33121123_1
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_RcCHAD
Plasmid#127782PurposeExpresses RcCHAD in E. coli cellsDepositorInsertRcCHAD
Tags6xHis tag + TEV cleavage siteExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC4785
Plasmid#113409PurposeTranslation reporter with inducible translation coupling (low native GFP expression, high under coupling)DepositorInsert359_31121113_2
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastPromoterT7Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG608
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
Tags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Med15
Plasmid#231887PurposeBacterial expression of N-terminally 6His tagged Gcn4 Med15DepositorInsertMed15
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0548
Plasmid#162299PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-TrxA-[TEV cleavage site]
UseSynthetic BiologyMutationcodon optimized for E. coliAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0550
Plasmid#162301PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-SUMO-[TEV cleavage site]
UseSynthetic BiologyAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0551
Plasmid#162303PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
UseSynthetic BiologyAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0547
Plasmid#162298PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, TrxA-[TEV cleavage site]
UseSynthetic BiologyMutationcodon optimized for E. coliAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0546
Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyMutationcodon optimized for E. coliAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0543
Plasmid#162294PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[TEV cleavage site]
UseSynthetic BiologyMutationcodon optimized for E. coliAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663K
Plasmid#92239PurposeExpress R663K mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663K mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to LysinePromoterT7 promoterAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-W695del
Plasmid#92241PurposeExpress W695-deletion mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-W695deletion mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationTryptophan 695 deletedPromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663Q
Plasmid#92240PurposeExpress R663Q mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663Q mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to GlutaminePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only