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Plasmid#112515PurposeProA driven gfpmut3 expression with synTTDepositorInsertProA gfpmut3 TT
UseTagsExpressionBacterialMutationPromoterProAAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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CD4-C-mCherry HDRT Source (pTR 175)
Plasmid#112019PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherryDepositorInsertCD4-mCherry HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBABE Rad C249
Plasmid#11215DepositorInsertRad (RRAD Human)
UseRetroviralTagsExpressionMammalianMutationC249: deletion of amino acids 248-308PromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE Rad N88
Plasmid#11218DepositorInsertRad (RRAD Human)
UseRetroviralTagsExpressionMammalianMutationN88: deletion of amino acids 1-88PromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE Rad PVQH
Plasmid#11223DepositorInsertRad (RRAD Human)
UseRetroviralTagsExpressionMammalianMutationPVQH: P101V/Q149HPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-bio-myc-cCE
Plasmid#112711PurposeFor tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsHIV Rev nuclear localization signal (NES), Stop c…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMV with Tet repressor binding sitesAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bio-myc-mCE 211-597
Plasmid#112716PurposeExpresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsbiotinylation signal peptide (Tagwerker et al. 20…ExpressionMammalianMutationdeleted amino acids 2-210 (triphosphatase domain)PromoterCMVAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c_S(-31)-M30_dT7term
Plasmid#112253PurposeExpresses the S(-31)-M30 apta-FRET RNA origami structure.DepositorInsertS(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28c_S*5-M5_dT7term
Plasmid#112254PurposeExpresses the S*5-M5 apta-FRET RNA origami structure.DepositorInsertS*5-M5 apta-FRET RNA origami with T7 promoter and terminators.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRNATU6.1 HDAC3 shRNA1
Plasmid#112249PurposeHDAC3 KDDepositorInsertshRNA against mouse HDAC3 (Hdac3 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. 3xFlag:PP4-19c[D85NH115N]
Plasmid#112247Purposeexpression of fly mutant PP4DepositorInsertPP4 (Pp4-19C Fly)
UseTags3xFLAGExpressionMammalianMutationD85NH115NPromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRNATU6.1 HDAC3 shRNA4neg
Plasmid#112273PurposeshRNA negative controlDepositorInsertshRNA negative control
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBoL1P3GA
Plasmid#112909PurposeExpress a cloned sgRNA from AtU626 promoterDepositorInsertAtU626-LACZ-sgRNA
UseTagsExpressionMutationPromoterAt U626Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBoL1P4GA
Plasmid#112910PurposeExpress a cloned sgRNA from AtU626 promoterDepositorInsertAtU626 PROMOTER-Laz-sgRNA
UseTagsExpressionMutationPromoterAtU626Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Adeno-miR-23b
Plasmid#112538PurposeAdenovirus overexpressionDepositorInsertmiR-23b (MIR23B Human)
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-TFSumo
Plasmid#112576PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TFSumo and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-Tsf
Plasmid#112578PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-Tsf and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-AFV
Plasmid#112560PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-AFV and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
peDK.11
Plasmid#112150Purpose"Vector DK"DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-5U6-sgRNAs-hsyn-EGFP
Plasmid#112213PurposeTargeted DNA methylationDepositorInsertsgRNAs
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K
Plasmid#112652PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K
UseTagsExpressionYeastMutationR59K (GAP-dead)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔC
Plasmid#112653PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔC
UseTagsExpressionYeastMutationR59K, Δ431-493 (deletion of C-terminal amphipathi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔGRM-ΔC
Plasmid#112654PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔGRM ΔC
UseTagsExpressionYeastMutationR59K, Δ376-493 (deletion of Glo3 motif & C-te…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K Δ2x+ve
Plasmid#112655PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K Δ2x+ve
UseTagsExpressionYeastMutationR59K, mutations (R223E, K224E, K225E, K233E, K234…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 A
Plasmid#112656PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398A
UseTagsExpressionYeastMutationR59K, mutations (S389A, S398A) in Glo3 motif, non…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 D
Plasmid#112657PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398D
UseTagsExpressionYeastMutationR59K, mutations (S389D, S398D) in Glo3 motif, pho…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
UseTagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 A
Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
UseTagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
UseTagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K
Plasmid#112647PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K
UseTagsExpressionYeastMutationR54KPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K L246D
Plasmid#112648PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K L246D
UseTagsExpressionYeastMutationR54K & L246D (in ALPS motif)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
UseTagsExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 Δ3xF AxxA
Plasmid#112650PurposeExpression S.cerevisiaeDepositorInsertGcs1 Δ3xF AxxA
UseTagsExpressionYeastMutationR54K, W304A, W307A, W310A, W349A, F353APromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF695.1.0-gDNA
Plasmid#112445PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF695DepositorInsertZNF695 (ZNF695 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-PRM-3R
Plasmid#112156PurposeMammalian eGFP expression plasmid for PRM-3R.DepositorInsertPRM-3R (ABL1 Human)
UseTagsExpressionMammalianMutationcontains only the PRM domain of ABl1PromoterCMVAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NFIX2ERT2
Plasmid#112704PurposeExpression of NFIX2-ERT2 fusion proteinDepositorInsertNuclear Factor I X2 (Nfix Mouse)
UseTagsERT2 and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[Tra].1026G
Plasmid#112689Purposeexpress gRNA targeting Tra under dU6-3 promoterDepositorInsertU6.3-gRNA[Tra] (tra Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[DsxF].1026A
Plasmid#112690Purposeexpress gRNA targeting DsxF under dU6-3 promoterDepositorInsertU6.3-gRNA[DsxF] (dsx Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNAs[bTub+Tra].1046D
Plasmid#112693Purposeexpress two gRNA targeting bTub & Tra under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub+DsxF].1046A
Plasmid#112694Purposeexpress two gRNA targeting bTub & DsxF under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF174-donor
Plasmid#112301PurposeCRISPR donor plasmid to tag human transcription factor ZNF174 with GFPDepositorInsertZNF174 homology arms flanking EGFP-IRES-Neo cassette (ZNF174 Human)
UseCRISPRTagsExpressionMutationrs37813, rs39729PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF57-donor
Plasmid#112296PurposeCRISPR donor plasmid to tag human transcription factor ZNF57 with GFPDepositorInsertZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)
UseCRISPRTagsExpressionMutationrs2288959, rs2288958, rs10410539, hg38:19:2918211…PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF257-donor
Plasmid#112294PurposeCRISPR donor plasmid to tag human transcription factor ZNF257 with GFPDepositorInsertZNF257 homology arms flanking EGFP-IRES-Neo cassette (ZNF257 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF778-donor
Plasmid#112379PurposeCRISPR donor plasmid to tag human transcription factor ZNF778 with GFPDepositorInsertZNF778 homology arms flanking EGFP-IRES-Neo cassette (ZNF778 Human)
UseCRISPRTagsExpressionMutationrs4785627,rs28415940,rs9921361,rs28638280,rs60437…PromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only