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Plasmid#185131PurposeSwi6 full length with L->A mutated in nuclear localization signalDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSWI6 deletion of amino acids 450-458PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB556
Plasmid#185108Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160ADepositorInsertPOM152
UseTagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB553
Plasmid#185107Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182LDepositorInsertPOM152
UseTagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB439
Plasmid#185093PurposeNMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::NMA111-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB345
Plasmid#185078Purposerfa3D46 truncation fused to GFPDepositorInsertRFA3
UseTagsGFPExpressionYeastMutationRfa3 truncation aa 1-46PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB419
Plasmid#185089PurposeSwi6L-GFP expressing N-term 273 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation at aa 273PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB462
Plasmid#185095PurposeNMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::nma111Dnls1Dnls2-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB354
Plasmid#185082PurposeSwi6L-GFP expressin N-term 273 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation aa 1-273PromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB416
Plasmid#185087PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB394
Plasmid#185086PurposeNMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNma111 KKR 9-11 AAA; KRK 28-30 AAAPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB349
Plasmid#185080Purposerfa2D248 truncation fused to GFPDepositorInsertRFA2
UseTagsGFPExpressionYeastMutationRfa2 truncation aa 1-247PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB353
Plasmid#185081PurposeSwi6M-GFP expressing N-term 181 amino acids of Swi6DepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 truncation aa 1-181PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB484
Plasmid#185132PurposeSWI6-GFP full length under control of GAL1 promotionDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CCG 316
Plasmid#131166PurposeFor ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Spt2-K166R-GFP
Plasmid#115573PurposeExpresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166DepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationLysine 166 mutated to ArgininePromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpGPD1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsVenusExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJLI-Sup35N-KDG6-C
Plasmid#1238DepositorInsertSUP35 (SUP35 Budding Yeast)
UseYeast integrative plasmidTagsExpressionMutationSUP35 middle region (aa124-253) was replaced with…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMK419 (GAL-OsTIR1(F74G))
Plasmid#140656PurposeGAL-OsTIR1(F74G)DepositorInsertGAL-OsTIR1(F74G)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE PGK-GFP-IRES-LUC-W
Plasmid#46793Purpose2nd generation lentiviral vectorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK198 (GAL-OsTIR1)
Plasmid#140655PurposeGAL-OsTIR1(WT)DepositorInsertOsTIR1(WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BAS286 C terminal SF-GFP hygBR
Plasmid#74081PurposeS.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistancDepositorInsertsuper-folder GFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLUM
Plasmid#40276DepositorUseTagsExpressionYeastMutationPromoterendogenous LEU2, endogenous MET15/17, and endogen…Available sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSin-GFP-Fg
Plasmid#174307PurposeLentiviral expression of GFPDepositorInsertGFP (eGFP Aequorea victoria)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
BAS285 C terminal mKO2 hygBR
Plasmid#74080PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertmKO2
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-NLS
Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Htb2
Plasmid#51569PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsHTB2 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BAS288 C terminal mKO2 natR
Plasmid#74083PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.DepositorInsertmKO2
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-PEX3
Plasmid#51572PurposeConstitutively express peroxisome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsPEX3 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
BAS292 C terminal SF-GFP kanR
Plasmid#74087PurposeS.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistanceDepositorInsertSF-GFP recoded for S.pombe
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
BAS287 C terminal E2C hygBR
Plasmid#74082PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertE2Crimson
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SIK1
Plasmid#51577PurposeConstitutively express nucleolus localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSIK1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-MYO1
Plasmid#51570PurposeConstitutively express myosin ring localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsMYO1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH353-SUP45wt
Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationWild type gene including genomic sequence 1432 bp…PromoterAvailable sinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
BAS289 C terminal E2C G418R
Plasmid#74084PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistanceDepositorInsertE2Crimson
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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BAS291 C terminal mKO2 kanR
Plasmid#74086PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.DepositorInsertmKO2
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAS290 C terminal E2C natR
Plasmid#74085PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistanceDepositorInsertE2Crimson
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH104
Plasmid#48262PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.DepositorInsertsTEF1p 5' fragment
URA3 5' fragment
TEF1p 3' fragment
UseRoutine cloning vectorTagsExpressionMutationFrom SK1 background. -343 to -1 relative to TEF1 …PromoterTEF1pAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
KBB430
Plasmid#185091PurposeNMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationFirst nuclear localization signal mutated (amino …PromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB438
Plasmid#185092PurposeGAL1::Rfa1[1-337, 344-621]-GFP with NLS removedDepositorInsertRFA1
UseTagsExpressionYeastMutationRfa1 with amino acids 338-343 deletedPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB372
Plasmid#185084PurposeGAL::Rfa1[330-621]-GFP under control of GAL1 promoterDepositorInsertRFA1
UseTagsGFPExpressionYeastMutationRfa1 amino acids 330-621PromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB428
Plasmid#185090PurposeNMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationFirst nuclear localization signal mutated (amino …PromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTJK513
Plasmid#121470PurposeCEN LEU2 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK516
Plasmid#121473PurposeCEN LEU2 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only