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pFUW-TetO-ZNF267

Plasmid

#178452

Purpose

doxycycline-inducible expression of Human ZNF267 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZNF267 (ZNF267 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-PLEK

Plasmid

#178439

Purpose

doxycycline-inducible expression of Human PLEK in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

PLEK (PLEK Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Junb

Plasmid

#178441

Purpose

doxycycline-inducible expression of Mouse Junb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Junb (Junb Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGPN-TMPRSS2

Plasmid

#187484

Purpose

Expression vector - TMPRSS2

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TFEC

Plasmid

#178447

Purpose

doxycycline-inducible expression of Human TFEC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

TFEC (TFEC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZFP36

Plasmid

#178438

Purpose

doxycycline-inducible expression of Human ZFP36 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZFP36 (ZFP36 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET a sfMatryoshCaMP6s-T78H

Plasmid

#100021

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfMatryoshCaMP6s

Use

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP and T78H m…

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-Ty1

Plasmid

#194588

Purpose

Bacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

Insert

Ty1 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NMI

Plasmid

#178446

Purpose

doxycycline-inducible expression of Human NMI in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

NMI (NMI Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-BATF2

Plasmid

#178453

Purpose

doxycycline-inducible expression of Human BATF2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

BATF2 (BATF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Irf7

Plasmid

#178445

Purpose

doxycycline-inducible expression of Mouse Irf7 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Irf7 (Irf7 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMP71-HLA-A0201-His

Plasmid

#108214

Purpose

HLA-A0201 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-A Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Expression

Mutation

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMA7-SacB

Plasmid

#79968

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Article

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase
Levansucrase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

Plasmid

#208041

Purpose

Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

TurboID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-IRF5

Plasmid

#178450

Purpose

doxycycline-inducible expression of Human IRF5 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

IRF5 (IRF5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGPN-ACE2

Plasmid

#187483

Purpose

Expression vector - ACE2

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CoV2-Omicron BA.1 spike

Plasmid

#194602

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

Depositor

Ben Murrell

Article

Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

Insert

CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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