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We narrowed to 4,010 results for: SPL;

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Showing: 241 - 260 of 4010 results

  4. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  5. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-492918

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  13. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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  14. HA Display H3_A/Hong Kong/1/1968

    Plasmid
    #206506
    Purpose
    Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells
    Depositor
    IIya Finkelstein
    Insert
    H3_A/Hong Kong/1/1968
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    deleted amino acids 1651 to 1735
    Promoter
    CMV
    Available Since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206506-plasmid-map-sequence-id-408102

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  15. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid
    #223498
    Purpose
    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker
    Depositor
    James Frank
    Insert
    HaloTag and EGFP
    Expression
    Mammalian
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    223498-plasmid-map-sequence-id-443020

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  17. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid
    #223503
    Purpose
    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag and EGFP
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223503-plasmid-map-sequence-id-443301

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Showing: 241 - 260 of 4010 results