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pRF-IcaR

Plasmid

#49362

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-NS

Plasmid

#140874

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mScarlet-I

Plasmid

#138391

Purpose

RMCE vector for generating C-terminal mScarlet-I fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mScarlet-I

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

Plasmid

#194463

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_5xUAS:EB3-mScarlet-I

Plasmid

#192358

Purpose

EB3 tagged with mScarlet-I, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

EB3 (MAPRE3 Human)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAR-IF reporter

Plasmid

#16628

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

CAR-IF

Use

Tags

beta-galactosidase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Use

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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H11-mScarletALG8

Plasmid

#172440

Purpose

Hipp11 targeting vector, expresses mScarlet-mALG8

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mALG8

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Scarlet-I

Plasmid

#193554

Purpose

Expresses mEGFP-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mScarlet-I

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCAR-OF reporter

Plasmid

#16627

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

CAR-OF

Use

Tags

beta-galactosidase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac-HS-NLRC4Δcard

Plasmid

#177276

Purpose

Purification of mouse NLRC4 (90-1024) from insect cells

Depositor

Hao Wu

Article

Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

Insert

NLRC4 (Nlrc4 Mouse)

Use

Tags

HIS-SUMO-PreScission

Expression

Insect

Mutation

I586T, N761S, M885V, K915R and F972L- please see …

Promoter

polyhedrin

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCardinal-Rab4a-7

Plasmid

#56174

Purpose

Localization: Ras GTPase, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Rab4a (RAB4A Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p3XFlag-CARMIL2

Plasmid

#118740

Purpose

To express CARMIL2 with Flag tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I-SYFP2

Plasmid

#189761

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1Q90A

Plasmid

#133249

Purpose

Express FLAG-tagged MCART1Q90A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, Q9…

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1R182A

Plasmid

#133252

Purpose

Express FLAG-tagged MCART1R182A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, R1…

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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cARA12-mRFP

Plasmid

#181961

Purpose

ARA12 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ARA12 (ARA12 Mustard Weed)

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

TBA2

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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mCardinal-CytERM-N-17

Plasmid

#56158

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CytERM

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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