We narrowed to 6,165 results for: cas9 expression plasmid

1. pdCas9-M-1D4

   Plasmid

   #73433

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1D4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-M-1E4

   Plasmid

   #73436

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1E4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-M-5F5

   Plasmid

   #73432

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 5F5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQdCas9-sggfp

   Plasmid

   #236184

   Purpose

   The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Quorum sensing promoter

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)_NoFlag_hROSA26_Left

   Plasmid

   #191442

   Purpose

   Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hROSA26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

   Plasmid

   #211767

   Purpose

   dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pGK and pEF1a

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti spCas9 T2A iRFP670 P2A puro

   Plasmid

   #122182

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   iRFP670
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid

    #122183

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    mNeonGreen
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid

    #196722

    Purpose

    Tet-inducible expression of Cas9-T2A-miRFP670nano

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid

    #160618

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid

    #160640

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Use

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge