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pETM33_Orf9b

Plasmid

#156485

Purpose

Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Orf9b

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET14b_tev_PSGU

Plasmid

#59738

Purpose

Expresses a.a. 417-887 of human ZO-1

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

zonula occludens-1 (TJP1 Human)

Use

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
ELF5_pet28a

Plasmid

#131659

Purpose

Bacterial expression of ETS gene ELF5

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF5 (ELF5 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pETM33_AP2B1_adaptin

Plasmid

#178467

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

AP2B1_adaptin (AP2B1 Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3b_ADRP

Plasmid

#156468

Purpose

Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3b_ADRP (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pET22b_PfSSB

Plasmid

#103000

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB

Depositor

Martin Webb

Article

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_CEP55_EABR

Plasmid

#178469

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CEP55_EABR (CEP55 Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3a_Ub1

Plasmid

#156467

Purpose

Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ETV7_pet28a

Plasmid

#131656

Purpose

Bacterial expression of ETS gene ETV7

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 (ETV7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD102

Plasmid

#131403

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD102

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1_CoiC_CoiSA

Plasmid

#208762

Purpose

Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiC
CoiSA

Use

Tags

Expression

Bacterial

Mutation

single nucleotide insertion (C70) at RBS of CoiC

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_FL

Plasmid

#59392

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ELF1_pet28a

Plasmid

#131649

Purpose

Bacterial expression of ETS gene ELF1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF1 (ELF1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM41_KPNB1_HEAT

Plasmid

#178494

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNB1_HEAT (KPNB1 Synthetic, Human)

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - GR

Plasmid

#89614

Purpose

Expresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Glutaredoxin

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6_Pfrm_BsMdh_BmHps_BmPhi

Plasmid

#129109

Purpose

Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

Depositor

Eleftherios Terry Papoutsakis

Article

Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

Insert

BsMDH BmHPS BmPHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pETM33_PTK2_Focal-AT

Plasmid

#178485

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PTK2_Focal-AT (PTK2 Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge