We narrowed to 6,905 results for: crispr cas9 plasmids

1. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

   Plasmid

   #188704

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   NFATC2 sgRNAs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121817

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121816

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121815

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121820

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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