We narrowed to 10,816 results for: HA

1. pTW195b63

   Plasmid

   #136931

   Purpose

   Directed evolution of SpCas11

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII(N)-npuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRES RED/SP M87C448Y

   Plasmid

   #89324

   Purpose

   Expression of RFP and M87C448Y spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 deleted

   Promoter

   CMV

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. paGFP-HRas

   Plasmid

   #18693

   Depositor

   Karel Svoboda

   Article

   Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   photoactivatable GFP

   Expression

   Mammalian

   Mutation

   A206K mutation in paGFP

   Promoter

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 S6K2 KR

   Plasmid

   #17730

   Depositor

   John Blenis

   Article

   Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

   Insert

   S6K2 (RPS6KB2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K99R: kinase dead mutant

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

5. NHA-hnRNPK

   Plasmid

   #120389

   Purpose

   Expression of HA tagged hnRNPK-lambdaN fusion

   Depositor

   Igor Ulitsky

   Article

   Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

   Insert

   hnRNPK (HNRNPK Human)
   

   Use

   Tags

   HA and LambdaN

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T7RNAP Cyslight V174C

   Plasmid

   #205078

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V174C

   Promoter

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCruzHA SIRT1 G261A

   Plasmid

   #10963

   Depositor

   Toren Finkel

   Article

   Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G261A

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pCGN-ELK-1 S383A

   Plasmid

   #27160

   Purpose

   For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   TAD mutant: S383A

   Promoter

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBlastR-12xLexAOp-mCherry-MiniWhite

   Plasmid

   #165917

   Purpose

   Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable LexAOp Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-miR-29b-1~29a

    Plasmid

    #51376

    Depositor

    Narry Kim

    Article

    Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

    Insert

    pri-miR-29b-1~29a (MIR29B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCGN-ELK-1 Ets mut

    Plasmid

    #27157

    Purpose

    For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R62E/R65E/Y66D

    Promoter

    Available Since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pTW194b63

    Plasmid

    #136930

    Purpose

    Directed evolution of SpCas10

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    npuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pINTO-N-FH

    Plasmid

    #65924

    Purpose

    Inducible expression of proteins fused to an N-terminal Flag-HA tag (FH)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2 (T-REx)

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Tag/WT M87

    Plasmid

    #89322

    Purpose

    expression of WT M87 spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-DIO {ChETA}on-WPRE

    Plasmid

    #111386

    Purpose

    AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Insert

    ChETA
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    humanized ChR2, E123T/H134R

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Tag/M1C448Y

    Plasmid

    #89319

    Purpose

    expression of M1 C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted, C448Y

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid

    #165907

    Purpose

    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P[acman]-Omega2

    Plasmid

    #165861

    Purpose

    Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Use

    Tags

    NES-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX-MAPKAP1.1-FHH-IRES-Puro

    Plasmid

    #120705

    Purpose

    Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hRad51(A190L-A192L)–Cas9(D10A)

    Plasmid

    #125562

    Purpose

    Expresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(A190L A192L)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid

    #165910

    Purpose

    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-Tag/M87 C448Y

    Plasmid

    #89320

    Purpose

    expression of M87 C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTRE/WT SP with 5'UTR

    Plasmid

    #89330

    Purpose

    Inducible expression of WT spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive P tight

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR'-ROMK1-CITE-GFP

    Plasmid

    #21322

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

    Insert

    ROMK1 (Kcnj1 Rat)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pR6Kg-Alpha1spm

    Plasmid

    #165866

    Purpose

    Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBlastR-5xUAS-mCherry-MiniWhite

    Plasmid

    #165911

    Purpose

    Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-FLAG-Lin28BdelN70

    Plasmid

    #51374

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28B (LIN28B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal 70 amino acids deletion

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pTrichoGate-13

    Plasmid

    #160713

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pR6Kg-Omega2

    Plasmid

    #165865

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCGN-ELK-1 R mut

    Plasmid

    #27159

    Purpose

    For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R box mutant: K249A/V250A/E251A

    Promoter

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pTRE/SP M1WT

    Plasmid

    #89327

    Purpose

    Inducible expression of WT M1 spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted

    Promoter

    Tet-responsive P tight

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTRE/SP M87 WT

    Plasmid

    #89328

    Purpose

    Inducible expression of WT M87 spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted

    Promoter

    Tet-responsive P tight

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBlastR-12xLexAOp-EBFP2-MiniWhite

    Plasmid

    #165918

    Purpose

    Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. hRad51(G151D)–Cas9(D10A)

    Plasmid

    #125565

    Purpose

    Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(G151D)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLEX-FHH-Raf1RBD

    Plasmid

    #120707

    Purpose

    Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Raf1 RBD
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Raf1 amino acids 1-149

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hRad51(F86E)–Cas9(D10A)

    Plasmid

    #125564

    Purpose

    Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(F86E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFP122

    Plasmid

    #21669

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pMCh-1468

    Plasmid

    #82418

    Purpose

    Plasmid for expression of HA-tagged Nematostella vectensis GW182 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pME_Cp_0_5a_005_HA-tag

    Plasmid

    #235887

    Purpose

    HA C-tag

    Depositor

    Tobias Erb

    Insert

    HA C-tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge