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TAL3072
Plasmid#41246DepositorInsertZebrafishCommunity-fan1-Left (si:ch211-201b11.2 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
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TAL3073
Plasmid#41247DepositorInsertZebrafishCommunity-fan1-Right (si:ch211-201b11.2 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
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GDF2_R (TAL 3011)
Plasmid#36003DepositorInsertTAL array targeting GDF2 (gdf2 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
GDF2_L (TAL 3010)
Plasmid#36002DepositorInsertTAL array targeting GDF2 (gdf2 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
MZB164
Plasmid#217842Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expressionDepositorInsertHaloTag
UseTags10x His, HaloTag, and SUMO (SMT3)ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTM-NiV-L
Plasmid#234657PurposeExpresses L gene of NiV Bangladesh strainDepositorInsertNiV L gene (L Nipah virus)
UseIn cell lines expressing bacteriophage t7 rna pol…TagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTM-NiV-P
Plasmid#234656PurposeExpresses P gene of NiV Bangladesh strainDepositorInsertNiV P gene (Nvgp2 Nipah virus)
UseIn cell lines expressing bacteriophage t7 rna pol…TagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTM-NiV-N
Plasmid#234655PurposeExpresses N gene of NiV Bangladesh strainDepositorInsertNiV N gene (N Nipah virus)
UseIn cell lines expressing bacteriophage t7 rna pol…TagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-NiV L(2k)-mCherry/eGFP
Plasmid#234654PurposeMinigenome plasmid of NiV Bangladesh strainDepositorInsertspart L gene
mCherry
eGFP
UseIn cell lines expressing bacteriophage t7 rna pol…TagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 solvvol W
Plasmid#231878PurposeBacterial expression of N-terminally 6His tagged Gcn4 solvvol WDepositorInsertGcn4 solvvol W
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 solvvol
Plasmid#231877PurposeBacterial expression of N-terminally 6His tagged Gcn4 solvvolDepositorInsertGcn4 solvvol
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 SATtoG KtoR
Plasmid#231861PurposeBacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoRDepositorInsertGcn4 SATtoG KtoR
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINF HopF2
Plasmid#228397PurposeRecombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coliDepositorInsertHopF2 (hopF2 Pseudomonas syringae pv. tomato)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-CC-S2
Plasmid#233243PurposeBacterial expression of Avi-CC-S2DepositorInsertAviTag-CC-S2
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneUseTagsHis6 and Twin-Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneUseTagsStrep-tag IIExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif3A-EGFP-SNAP
Plasmid#229852PurposeKif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif3A (Kif3a Mouse)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLANT-2b
Plasmid#231487PurposeExpresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.DepositorInsertsUseTagsHis6 tag and His6-SUMO^NITExpressionBacterialMutationDeleted amino acids 1-6 (MEPEES). and M83L, E84Q,…PromoterT7 promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcRII-mogat3a in situ probe
Plasmid#232135Purposezebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat3a
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySUMO3
Plasmid#221067PurposeExpression of polySUMO3 protein under T7 promoterDepositorInsertFive repeats of SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-BASU-His
Plasmid#218451PurposeE coli expression vector for Halo-BASU-His biotin ligaseDepositorInsertHaloTag-BASU-His fusion protein
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB216
Plasmid#221766Purpose"Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorInsertrtxA
UseTagsExpressionMutationPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
E547* FL USP7
Plasmid#229001PurposeE547-mutant of human USP7DepositorInsertUSP7 1-546 (USP7 Human)
UseTags6xHis cleavable with thrombinExpressionBacterialMutationE547 mutated to stop codonPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(CysCNP)RS
Plasmid#225054PurposetRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codonDepositorArticleInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF53
Plasmid#229477Purposeexpresses RssBE229CDepositorInsertRssB (E229C)
UseTagsExpressionBacterialMutationE229CPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD1
Plasmid#229463PurposeExpresses RssBDepositorInsertRssB
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only
