We narrowed to 10,761 results for: T7

Showing: 5161 - 5200 of 10761 results

Results per page:

Sort results by:

pT7-stl

Plasmid

#51721

Purpose

conditional gene knockdowns in Trypanosoma brucei

Depositor

Arthur Gunzl

Article

Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

Type

Empty backbone

Use

Conditional gene knockdown in t. brucei

Promoter

T7 (2X)

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 GTF2H2D

Plasmid

#67081

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H2D (GTF2H2C_2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDM_noro_A1_lacZA

Plasmid

#118818

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A1

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3144

Plasmid

#41318

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Left (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3145

Plasmid

#41319

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Right (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3200

Plasmid

#41374

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3201

Plasmid

#41375

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pZF-ADD-GCN

Plasmid

#127475

Purpose

coding sequence for synthetic zinc finger ZF-ADD-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 RAP2A

Plasmid

#67058

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAP2A (RAP2A Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET11a-TAX-L10-STEPtag

Plasmid

#159375

Purpose

Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

TAX-L10-STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-STEPtag-L10-TAX

Plasmid

#159374

Purpose

Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag-L10-TAX

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b- hTPI Ile170Val

Plasmid

#50724

Purpose

overexpression of the human TPI1 Ile170Val allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Valine

Promoter

T7

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
dFP

Plasmid

#120542

Purpose

Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein (dFP)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET20b-CeNLuc-6xHis

Plasmid

#135933

Purpose

Expresses the CeNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-HtsptLB12v.3

Plasmid

#45123

Depositor

David Baker

Article

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

HtsptLB12v.3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
TAL3175

Plasmid

#41349

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-socs3b-Right (socs3b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pRC02

Plasmid

#114681

Purpose

sfGFP.Strand10-Haloenzyme

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP.Strand10-Haloenzyme

Tags

His10

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116956

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3250

Plasmid

#42713

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-esr1-left (esr1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3251

Plasmid

#42714

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-esr1-right (esr1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3366

Plasmid

#42829

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox1a-left (sox1a Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3367

Plasmid

#42830

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox1a-right (sox1a Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3124

Plasmid

#41298

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-mycn-Left (mycn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3125

Plasmid

#41299

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-mycn-Right (mycn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSC-hTDG-K330A-PreE2

Plasmid

#52273

Purpose

Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

Ubc9-GST

Expression

Bacterial

Mutation

K330A, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3131

Plasmid

#41305

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nr2e3-Right (nr2e3 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
TAL3130

Plasmid

#41304

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nr2e3-Left (nr2e3 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
ptp4a3_L (TAL 3000)

Plasmid

#35992

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting ptp4a3 (ptp4a3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-clbH

Plasmid

#114156

Purpose

Expresses full-length ClbH

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB326

Plasmid

#113670

Purpose

expresses Ulp1_R2 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R2

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pST96/LBT-MCS

Plasmid

#108227

Purpose

Bacterial expression expression of protein with N-terminal lanthanide binding tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT

Expression

Bacterial

Promoter

T7, lacO

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b/GgVcl V1-258 (aka VD1)

Plasmid

#46173

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-258 (VCL Chicken)

Tags

His

Expression

Bacterial

Mutation

aa 1-258 (VD1)

Promoter

T7

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3563

Plasmid

#47282

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stxbp1a-right (stxbp1a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHYDuet93

Plasmid

#42303

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaEdC6

Tags

H6-GB1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3349

Plasmid

#42812

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-Purinergic receptor Y2-right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Article

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
Stim1-CaRQ

Plasmid

#34560

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

Stim1-CaRQ (STIM1 Human)

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

Showing: 5161 - 5200 of 10761 results

Results per page:

Sort results by:

More Filters

We narrowed to 10,761 results for: T7