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pKnockinDonor-humanCTCF-miniAID-mClover3

Plasmid

#136883

Purpose

knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

Depositor

Chunliang Li

Article

Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

Insert

CTCF-knockin-homology arm-miniAID-mClover3

Tags

miniAID-mClover3

Expression

Mammalian

Promoter

no promoter

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Actin-C-18

Plasmid

#158009

Purpose

Visualization of actin in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Actin (ACTB Human)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_2

Plasmid

#102971

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_2 (FXN Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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EYFP-NLS-actin-K68/284R

Plasmid

#60616

Purpose

Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

K68R, 284R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pMyc1ElbLuc,

Plasmid

#53243

Purpose

reporter plasmid containing 1 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc1ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pXPR_003 sgMYC guide 2

Plasmid

#193597

Purpose

cMYC knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMYC guide 2 (MYC Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LAP2-beta-actin in modified pEGFP

Plasmid

#34839

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LAP2-beta-Actin (ACTB Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
pACEBac1-beta-actin

Plasmid

#178076

Purpose

Insect cell expression of beta-actin

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

beta-actin (ACTB Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCINeo-hFX-HA

Plasmid

#14976

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
SI16-Kit a

Plasmid

#58948

Purpose

expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Kit receptor a mouse monoclonal antibody (kita Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-mCh-ACTB-P2A-EGFP

Plasmid

#165158

Purpose

Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

Depositor

Taekjip Ha

Article

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

mCherry-ACTB-P2A-EGFP (ACTB Human)

Use

Retroviral

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Beclin1 (1-242)

Plasmid

#24401

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 1-242 only.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-7

Plasmid

#55399

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
MDH1-miR-181a1-Dusp6

Plasmid

#17849

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

Dusp6-EGFP (Dusp6 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pISH-Hopx

Plasmid

#74347

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

HOP homeobox (Hopx Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-7

Plasmid

#14968

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-26/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-26/+334 (relative to the P2 transcription initia…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

John Penniston, Emanuel Strehler

Article

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_CLTC_Clathrin

Plasmid

#109997

Purpose

Protein expression and purification of CLTC_Clathrin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CLTC_Clathrin (CLTC Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only