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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,268 results for: SUB

Showing: 6501 - 6550 of 9268 results

  1. pJK521

    Plasmid
    #72440
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Unspecified
    Tags
    Expression
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72440-plasmid-map-sequence-id-133778

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  2. pJK536

    Plasmid
    #72444
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72444-plasmid-map-sequence-id-136729

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  3. Pv-RNR-R2

    Plasmid
    #25589
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-RNR-R2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pBS-L1PA1CH blast

    Plasmid
    #69612
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Use
    Tags
    blast cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69612-plasmid-map-sequence-id-123345

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  7. pBS-L1PA13ACHmneo

    Plasmid
    #69613
    Purpose
    Expresses a neomycin tagged codon optimized L1PA13A
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA13A
    Use
    Tags
    mneo retrotransposition cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69613-plasmid-map-sequence-id-123346

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  8. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

    Plasmid
    #86454
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86454-plasmid-map-sequence-id-170900

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  9. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Georg Ramm
    Insert
    LOC8R
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66896-plasmid-map-sequence-id-114924

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  10. pMH_AtCK2beta1_95-287_NoTag

    Plasmid
    #238520
    Purpose
    Bacterial expression of A. thaliana CK2beta1 95-287_NoTag
    Depositor
    Michael Hothorn
    Insert
    AtCK2beta1 (CKB1 Mustard Weed)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only

  11. Ec dnaX(T-only)-pET(3c)

    Plasmid
    #237237
    Purpose
    over-express E. coli PolX (Pol III tau subunit - no gamma)
    Depositor
    Michael O'Donnell
    Insert
    dnaX (tau only) (dnaX E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237237-plasmid-map-sequence-id-469257

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  12. Ec dnaN-pET(11a)

    Plasmid
    #237238
    Purpose
    over-express E. coli dnaN (Pol III beta sliding clamp subunit)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta) (dnaN E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237238-plasmid-map-sequence-id-469258

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  13. Ec holA-pET(11a)(ACH-)

    Plasmid
    #237233
    Purpose
    over-express E. coli holA (Pol III delta subunit)
    Depositor
    Michael O'Donnell
    Insert
    holA (delta) (holA E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237233-plasmid-map-sequence-id-469254

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  14. pAIP4_3OBS

    Plasmid
    #234227
    Purpose
    Heterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3OBS (TSG101 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234227-plasmid-map-sequence-id-464192

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  15. pAIP4_2WWE

    Plasmid
    #234210
    Purpose
    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2WWE (PIK3C2G Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234210-plasmid-map-sequence-id-464175

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  16. Kv1.3/RBG4

    Plasmid
    #196949
    Purpose
    Plasmid expressing an antigen targeting the Kv1.3 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    Kv1.3 (Kcna3 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196949-plasmid-map-sequence-id-388566

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  17. DsRed-CPI-17-T38A-NLS

    Plasmid
    #100792
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear
    Depositor
    Joseph Gordon
    Insert
    protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100792-plasmid-map-sequence-id-193236

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  18. pJK523

    Plasmid
    #72442
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72442-plasmid-map-sequence-id-133863

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  19. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  20. pRL528

    Plasmid
    #58495
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al Methods Enzymol. 1988;167:747-54.
    Insert
    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58495-plasmid-map-sequence-id-107317

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  21. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Use
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69611-plasmid-map-sequence-id-123344

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  22. pB2Hopt

    Plasmid
    #163830
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163830-plasmid-map-sequence-id-337586

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  25. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

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  28. pMH_MBP_AtCK2beta1_95-287

    Plasmid
    #238519
    Purpose
    Bacterial expression of A. thaliana CK2beta1 95-287
    Depositor
    Michael Hothorn
    Insert
    AtCK2beta1 (CKB1 Mustard Weed)
    Use
    Tags
    2x Strep, 6x His, MBP, N-term, TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only

  29. Ec holB-pET(11a)(ACH-)

    Plasmid
    #237234
    Purpose
    over-express E. coli holB (Pol III delta prime subunit)
    Depositor
    Michael O'Donnell
    Insert
    holB (delta prime) (holB E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237234-plasmid-map-sequence-id-469255

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  30. pAIP4_3ONH

    Plasmid
    #234179
    Purpose
    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ONH (UBA2 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234179-plasmid-map-sequence-id-464144

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  31. Kv1.2/RBG4

    Plasmid
    #196941
    Purpose
    Plasmid expressing an antigen targeting the Kv1.2 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    Kv1.2 (Kcna2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196941-plasmid-map-sequence-id-387871

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  32. Kv1.5/RBG4

    Plasmid
    #196945
    Purpose
    Plasmid expressing an antigen targeting the Kv1.5 potassium voltage-gated channel subunit
    Depositor
    James Trimmer
    Insert
    Kv1.5 (Kcna5 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196945-plasmid-map-sequence-id-387869

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  33. Kir2.1-HA/GW1

    Plasmid
    #197342
    Purpose
    Plasmid expressing an antigen targeting the Kir2.1 potassium channel subunit
    Depositor
    Dzwokai Ma
    Insert
    Kir2.1 (Kcnj2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197342-plasmid-map-sequence-id-388454

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  34. pB2H6

    Plasmid
    #163835
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163835-plasmid-map-sequence-id-337603

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  35. PP-RNR

    Plasmid
    #25135
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PY03671:C21955 (PY03671 Plasmodium yoelii)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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  37. HCN4/EGFP-C1

    Plasmid
    #197389
    Purpose
    Plasmid expressing an antigen targeting the HCN4 ion channel subunit
    Depositor
    Dane Chetkovich
    Insert
    HCN4 (HCN4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197389-plasmid-map-sequence-id-401571

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  38. HCN3/pCDNA3.1

    Plasmid
    #197293
    Purpose
    Plasmid expressing an antigen targeting the HCN3 ion channel subunit
    Depositor
    Dane Chetkovich
    Insert
    HCN3 (Hcn3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197293-plasmid-map-sequence-id-388075

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  39. BK beta1 ECFP

    Plasmid
    #113561
    Purpose
    mouse BK beta1 subunit in C-terminal translational frame with ECFP
    Depositor
    Robert Brenner
    Insert
    Kcnmb1 (Kcnmb1 Mouse)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113561-plasmid-map-sequence-id-218926

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  40. alpha5-EGFP

    Plasmid
    #118956
    Purpose
    GABAA receptor expression (alpha5 subunit). EGFP tag at the C-terminus.
    Depositor
    Angel de Blas
    Insert
    GABRA5 (Gabra5 Rat)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118956-plasmid-map-sequence-id-228584

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  41. Dynactin-4/p62-GFP

    Plasmid
    #31071
    Depositor
    Vann Bennett
    Insert
    Dynactin-4 (DCTN4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    31071-plasmid-map-sequence-id-203407

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  42. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  43. pDON-AI-Npm2(N533)

    Plasmid
    #51475
    Purpose
    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)
    Depositor
    Shunsuke Ishii
    Insert
    Nucleoplasmin 2 (Npm2 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …
    Promoter
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51475-plasmid-map-sequence-id-78547

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  44. KvB2/RBG4

    Plasmid
    #196942
    Purpose
    Plasmid expressing an antigen targeting the Kvbeta2 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    KvB2/B6 (Kcnab2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196942-plasmid-map-sequence-id-394742

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  46. pcDNA3+ hSlo2.2

    Plasmid
    #16214
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    This construct lacks the first 53aa when compared…
    Promoter
    Available since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only
    16214-plasmid-map-sequence-id-248256

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  47. BK beta2 EGFP

    Plasmid
    #113558
    Purpose
    human BK beta2 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb2 (KCNMB2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113558-plasmid-map-sequence-id-218921

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  48. KCNQ4-Myc

    Plasmid
    #197329
    Purpose
    Plasmid expressing an antigen targeting the KCNQ4/Kv7/4 K+ channel subunit
    Depositor
    Thomas Jespersen
    Insert
    KCNQ4 (KCNQ4 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197329-plasmid-map-sequence-id-406745

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  49. Myr-(iSH2-p85)-p110alpha-Myc

    Plasmid
    #1410
    Depositor
    Lewis Cantley
    Insert
    PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    SRalpha promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    1410-plasmid-map-sequence-id-352163

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  50. BK beta4 EGFP

    Plasmid
    #113560
    Purpose
    human BK beta4 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb4 (KCNMB4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113560-plasmid-map-sequence-id-218925

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Showing: 6501 - 6550 of 9268 results