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pDONR221-W279A-short-UBASH3A-Ctag

Plasmid

#195402

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Mutation

W279A

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ctag

Plasmid

#195399

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Mutation

K202R

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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K202R-short-UBASH3A-V5-6xHis

Plasmid

#195406

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

K202R

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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W279A-short-UBASH3A-V5-6xHis

Plasmid

#195409

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

W279A

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDL71

Plasmid

#175903

Purpose

pENTRY for Gateway LR reaction

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#28

Use

Pentry clone

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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S3_001_026_096

Plasmid

#134402

Purpose

pYTK096-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHL 1148

Plasmid

#53006

Purpose

pCon:OxyS, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

OxyS sRNA
sodB

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

contains −56 to +141 bp relative to start codon

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1070

Plasmid

#53002

Purpose

pCon:DsrA, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

DsrA sRNA
sodB

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

contains −56 to +141 bp relative to start codon

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1057

Plasmid

#53001

Purpose

pCon:RhyB, pLlacO-1:rpoS::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
rpoS

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

containsn −150 to +30 bp relative to start codon

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
HL 3076 (=HL 716 + pHL 1069)

Plasmid

#30036

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pCon:MicC, pLlacO-1:sodB::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
HL 4179 (=HL 865 + pHL 1381)

Plasmid

#30098

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:hfq, pLtetO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
HL 4202 (=HL 716 + pHL 1389)

Plasmid

#30100

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
HL 4178 (=HL 862 + pHL 1360)

Plasmid

#30097

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:hfq, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-Volton2-ST-WPRE

Plasmid

#172907

Purpose

AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Has Service

AAV1

Insert

Voltron2-ST

Use

AAV and Cre/Lox

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

hSynapsin1

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

Plasmid

#118162

Purpose

CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

Plasmid

#125430

Purpose

CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

Plasmid

#118169

Purpose

CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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