Addgene

Showing: 2621 - 2640 of 11705 results

Results per page:

Sort results by:

pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCDNA3 Flag MKK7a1

Plasmid

#14538

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+_V5-ANKRD52

Plasmid

#92012

Purpose

Expresses V5-ANKRD52

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

ANKRD52 (ANKRD52 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1 hSCN5A-L325R

Plasmid

#145373

Purpose

expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

Depositor

Hugues Abriel

Article

Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

Expression

Mammalian

Mutation

changed Leucine 325 to Arginine

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Use

Tags

N-terminal mScarlet tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO V5 DNAJB9

Plasmid

#19532

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Ha-Brca1 S1387A

Plasmid

#32532

Depositor

Michael Kastan

Article

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

Brca1 (BRCA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S1387A

Promoter

CMV/Rabbit B-Globin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Use

Tags

3xFlag, His, and T7

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA6.MCV.LT206.V5(CM2B4)

Plasmid

#28190

Depositor

Patrick Moore

Insert

MCPyV genomic T antigen locus (206 wild-type strain)

Use

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/V5-His-TN-DROSHA

Plasmid

#106252

Purpose

Express TN-DROSHA in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

Insert

Drosha Ribonuclease III (DROSHA Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

E1045Q and E1222Q mutations

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Myc-Smad8

Plasmid

#11745

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

SMAD8 (Smad9 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA4-Xpress-GBF1

Plasmid

#206417

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/TO_H2B_HaloTag9

Plasmid

#169334

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna DSG-2 Tailless

Plasmid

#89466

Purpose

DSG-2 tailless control

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pY018 (pcDNA3.1-hPdCpf1)

Plasmid

#69990

Purpose

Expresses humanized PdCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPdCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 EYFP H148Q

Plasmid

#25871

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q

Use

Tags

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with Histidine 148 to …

Promoter

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA TfR1-miniE

Plasmid

#181987

Purpose

MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

TfR1-MiniE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 proHB-EGF NC

Plasmid

#11605

Depositor

Rosalyn Adam

Article

Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

Insert

HB-EGF (HBEGF Human)

Use

Tags

Expression

Mammalian

Mutation

Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-RsSHC014-RBD-8h

Plasmid

#161826

Purpose

Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Receptor-binding domain (RBD) of spike protein S

Use

Tags

GSG linker and 8his tag and HA leader sequence

Expression

Mammalian

Mutation

Human codon optimized RBD (a.a. T321-D519)

Promoter

CMV

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only