Addgene

We narrowed to 11,176 results for: SHA

Showing: 2641 - 2660 of 11176 results

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pAAV-CAG-NIR-FbLAG30

Plasmid

#220750

Purpose

NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

AAV and Affinity Reagent/ Antibody

Tags

Expression

Mutation

Promoter

CAG

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNK6031

Plasmid

#219739

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCV

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

Cestrum yellow leaf curling virus (CmYLCV) promoter

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight-VP16

Plasmid

#219549

Purpose

NLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight-VP16

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 mCerulean

Plasmid

#216227

Purpose

TDP-43 with a C-terminal mCerulean tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI38-PRM-wasabi

Plasmid

#195554

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI38

Use

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pmcb306-GFP-Rab12-mito

Plasmid

#207349

Purpose

Mammalian expression of Rab12-mito

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab12-mito (RAB12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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eDrTrkB

Plasmid

#183997

Purpose

Optically controlled eDrTrkB expression in mammalian cells

Depositor

Vladislav Verkhusha, Dmitry Gerashchenko

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkB (NTRK2 Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP718nano

Plasmid

#195747

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-clathrin light chain

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP718nano

Plasmid

#195746

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-human alpha-tubulin

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-QPAS1-VP16

Plasmid

#186187

Purpose

AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

NLS-QPAS1-VP16

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-H2b-emiRFP670-P2A-TEVP

Plasmid

#174379

Purpose

AAV virus for expressing TEVP and H2b-emiRFP670

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TEVP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRV∆G-N*PEST-4Cre

Plasmid

#174381

Purpose

Rabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

Cre recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 ZUC

Plasmid

#169964

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT I261A

Plasmid

#153476

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT I261A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, I261A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT S333A

Plasmid

#153477

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT S333A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, S333A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin-TAPBPR 22-35

Plasmid

#153478

Purpose

Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin delta2step

Plasmid

#153479

Purpose

Mammalian expression of FLAG-tagged Tapasin delta2step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.13-14, 16-17

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin delta3step

Plasmid

#153480

Purpose

Mammalian expression of FLAG-tagged Tapasin delta3step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.12-14, 16-18

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin G15E

Plasmid

#153481

Purpose

Mammalian expression of FLAG-tagged Tapasin G15E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

G15E

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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