We narrowed to 26,603 results for: STI

1. pSEVA251::PpsbA2*::B0032::CYP110D1

   Plasmid

   #186707

   Purpose

   CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

   Depositor

   Paula Tamagnini

   Article

   Mascia et al Green Chemistry, 24 (2022):6156

   Insert

   alr4766
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PpsbA2*

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1

   Plasmid

   #186709

   Purpose

   CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

   Depositor

   Paula Tamagnini

   Article

   Mascia et al Green Chemistry, 24 (2022):6156

   Insert

   alr4766
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ptrc.x.tetO2

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1-AA027

   Plasmid

   #185752

   Purpose

   Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Insert

   HygR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnYC-NpM-DmPAN3_349-790-P652G_T

   Plasmid

   #147570

   Purpose

   Bacterial Expression of DmPAN3_349-790-P652G

   Depositor

   Elisa Izaurralde

   Article

   Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

   Insert

   DmPAN3_349-790-P652G (CG11486 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-GgVCL/WT

   Plasmid

   #162787

   Purpose

   CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Gallus gallus vinculin (VCL Chicken)
   

   Use

   Tags

   IRES-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   beta actin

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAID5WY

   Plasmid

   #165050

   Purpose

   Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Promoter

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFE-sfGFP

   Plasmid

   #162688

   Purpose

   pFE vector control expressing sfGFP

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   sfGFP
   

   Use

   Tags

   C terminal 6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

   Plasmid

   #162805

   Purpose

   STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI-HA_IRES_Blasticidin
   

   Use

   Piggybac

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2 8xSTAR-sTomato. PGK-puro

   Plasmid

   #136264

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV0747

    Plasmid

    #133481

    Purpose

    Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV0748

    Plasmid

    #133482

    Purpose

    Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV0605

    Plasmid

    #133503

    Purpose

    Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV0746

    Plasmid

    #133480

    Purpose

    Expression in fission yeast from promoter prga3 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSW377 - pTMO5

    Plasmid

    #115988

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pTMO5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DeltaNIRD C95S KVS-1

    Plasmid

    #113068

    Purpose

    Expresses worm truncated KVS1.2 C113S in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

    Insert

    C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C113S+first 40 AA deletion

    Promoter

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. CTL2 (M2225R)

    Plasmid

    #96952

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 (M222R) of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

    Plasmid

    #91945

    Purpose

    Bacterial expression of maize SAE1 and truncated SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Use

    Tags

    FLAG and HA

    Expression

    Bacterial

    Mutation

    Truncation of ZmSAE2 to mimic a splice variant

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Tag/S46X with 5'UTR

    Plasmid

    #87729

    Purpose

    Expresses S46X spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S46X

    Promoter

    CMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Tag/S44L with 5'UTR

    Plasmid

    #87727

    Purpose

    Expresses S44L spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S44L

    Promoter

    pCMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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