We narrowed to 21,628 results for: ESC

1. pMRE151

   Plasmid

   #118501

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICSL80036

   Plasmid

   #68259

   Purpose

   Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Coding sequence, hygromycin phophotransferase II (Escherichia coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDUAL_2_T5

   Plasmid

   #155175

   Purpose

   see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-syn-fRCaMP1

   Plasmid

   #125245

   Purpose

   Expresses red fluorescent calcium sensor in hippocampal slices

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRCaMP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W44Y

   Promoter

   synapsin

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR322-bom

   Plasmid

   #155178

   Purpose

   pBICI_2_strong without bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXGFPC-5 Pxyl-dcas9 (Spy)

   Plasmid

   #133316

   Purpose

   expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas12j3 VPR

   Plasmid

   #188517

   Purpose

   Expresses FLAG tagged dCas12j3 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas12j2 VPR

   Plasmid

   #188518

   Purpose

   Expresses FLAG tagged dCas12j2 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D394A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-NEW-fRGECO2

    Plasmid

    #125248

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4046925

    Plasmid

    #145694

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pTol1-UAS:CheRiff-tdTomato

    Plasmid

    #124234

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CheRiff-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLAMP1-miRFP718nano

    Plasmid

    #195749

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    LAMP1-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGST2-CeEP55

    Plasmid

    #21501

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    CEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Q230 (CAG changed to TAA), Q218 (CAA changed to T…

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pActin-miRFP718nano

    Plasmid

    #195745

    Purpose

    Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human beta-actin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. D3 clone (p21/WAF1 promoter)

    Plasmid

    #16452

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 promoter (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pBICI_2_strong

    Plasmid

    #155168

    Purpose

    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE157

    Plasmid

    #118507

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-CAG-DIO-mAPX

    Plasmid

    #79908

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    mAPX
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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