We narrowed to 16,829 results for: Emb;
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Plasmid#71712PurposeMembrane scaffold protein (MSP), half of helix 4 truncatedDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
myc-hIFITM3-S61A,N64A,T65A
Plasmid#104362PurposeExpresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationmutated the following amino acids: S61A,N64A,T65APromoterCMVAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HseIF4AII_AC
Plasmid#148439PurposeMammalian Expression of HseIF4AIIDepositorInsertHseIF4AII (EIF4A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_ERBB2
Plasmid#187776PurposeMAC-tagged gene expression of human ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Strep/HA-UBE2QL1
Plasmid#124665PurposeExpresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK601
Plasmid#110607PurposeBTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)DepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
NKP68 RFP (790)
Plasmid#62003Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNKP68 (TMUB1 Human)
UseTagsmRFPExpressionMammalianMutationPromoterpgkAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET5L pEGFP-N2 (1155)
Plasmid#62035Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET5L (TMEM201 Human)
UseTagsEGFPExpressionMammalianMutationlong formPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-BICD2N
Plasmid#64070PurposeN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)DepositorInsertBICD2N (1-400) (Bicd2 Mouse)
UseTagsGFP and His-ZZ-TCSExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lyn11C3S-EGFP
Plasmid#202747Purposeweakly plasma membrane targeted fluorescent proteinDepositorInsertLYN (1-11) (C3S):EGFP (LYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
POPDC2 pEGFP-N2 (1087)
Plasmid#62030Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertPOPDC2 (POPDC2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinExpressionMutationPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK165_Mem-mCherry-4.1G-CTD
Plasmid#46362DepositorInsert4.1G C-terminal domain (EPB41L2 Human)
UseTagsMem (N-terminal 20 amino acids of neuromodulin fo…ExpressionMammalianMutationC-terminal domain from 886-1005 amino acidsPromoterCMVAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-hBAI3-HormR-GAIN
Plasmid#37848DepositorInsertBAI3 (BAI3 Human)
UseBaculovirus transfer vectorsTags8xHISExpressionMutationHormR and GAIN domains only (aa498–868PromoterPolyhedrinAvailable sinceJuly 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Strep/HA-UBE2Q2
Plasmid#124668PurposeExpresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2Q2 (UBE2Q2 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only