We narrowed to 5,312 results for: cycs

1. pKHH030_sgAMBRA1#1

   Plasmid

   #174146

   Purpose

   Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-RPS14(WT)-Myc

   Plasmid

   #122236

   Purpose

   expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113699

   Purpose

   Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVZ_PcoaT_SQE1_THAS1

   Plasmid

   #103859

   Purpose

   Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803

   Depositor

   Ilka Axmann

   Article

   Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

   Insert

   thalianol synthase 1 (THAS1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   inserted genes are codon optimized. 2nd amino aci…

   Promoter

   PcoaT (from Synechocystis sp. PCC 6803)

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOEM1_pCMV:deltaN-bmp4-pPH:vsvged

   Plasmid

   #111158

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, hShh

   Depositor

   Elly Tanaka

   Article

   Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

   Insert

   deltaNhBmp4 (BMP4 Human)
   

   Use

   Baculoviral rescue shuttle vector

   Tags

   Expression

   Insect and Mammalian

   Mutation

   delta N, mutant BMP lacking the N terminus (delta…

   Promoter

   CMV

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVZ_PcoaT_SQE1_MRN1

   Plasmid

   #103858

   Purpose

   Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803

   Depositor

   Ilka Axmann

   Article

   Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

   Insert

   marneral synthase 1 (MRN1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   inserted genes are codon optimized2nd amino acid …

   Promoter

   PcoaT (from Synechocystis sp. PCC 6803)

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-CuR-hSOD1

   Plasmid

   #232477

   Purpose

   The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Homo sapiens superoxide dismutase 1 (SOD1 Human)
   

   Use

   Transposon system

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-CuO

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TRE-OKSM

   Plasmid

   #204498

   Purpose

   Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Inserts

   OCT4 (POU5F1 Human)
   KLF4 (KLF4 Human)
   SOX2 (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRIPZ-p21-HA(RxL)

    Plasmid

    #215111

    Purpose

    Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

    Promoter

    CMV (Tet inducible)

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct

    Plasmid

    #175552

    Purpose

    Targeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    SOX2 (Sox2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    FKBPV-HA2-P2A-mCherry

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTRIPZ-p21-HA(dPCNA)

    Plasmid

    #215112

    Purpose

    Expresses p21-HA(dPCNA) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    CMV (Tet inducible)

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-AMBRA1-501*-3xFLAG

    Plasmid

    #174160

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 501-1208

    Promoter

    EF1A

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-AMBRA1-217*-3xFLAG

    Plasmid

    #174161

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 217-1208

    Promoter

    EF1A

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pExp-His-zBasic-RBD

    Plasmid

    #194999

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli

    Depositor

    Marko Hyvönen

    Article

    Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8-Zbasic

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM

    Plasmid

    #186889

    Purpose

    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion (CGAS Synthetic, Pongo abelii)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p21(dPCNA)-mCerulean-NeoR

    Plasmid

    #215082

    Purpose

    Expressesp21(dPCNA)-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p21(RxL)-mCerulean-NeoR

    Plasmid

    #215081

    Purpose

    Expresses p21(RxL)-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p21(RxL)-mCerulean-PuroR

    Plasmid

    #215078

    Purpose

    Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge