We narrowed to 6,089 results for: nls
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pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Puro-Cas9 donor
Plasmid#58409PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locusDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-CNA35
Plasmid#61607Purposebacterial expression of N-terminal fusion protein of CNA35 with mCherryDepositorInsertmCherry and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-EGFP-CNA35
Plasmid#61603Purposebacterial expression of N-terminal fusion protein of CNA35 with EGFPDepositorInsertEGFP and CNA35
UseTagsHis tagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52
Plasmid#98678PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas3
Plasmid#178881PurposeExpression of human codon-optimized NlaCas3 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas3c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneUseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TATV) (pY220)
Plasmid#89353PurposeExpresses humanized AsCpf1 variant that recognizes TATV PAMsDepositorInsertCpf1, RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AktAR2
Plasmid#184049PurposeNuclear-targeted ExRai-AktAR2.DepositorInsertNuc-ExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_pCDest
Plasmid#119923PurposeNme2Cas9 CMV-driven mammalian expression plasmidDepositorInsertNme2Cas9
UseTagsNLS-3xHA-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-BFP
Plasmid#215720PurposeCatalytically inactive dCasRx with 2A-BFPDepositorInsertdCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP1830
Plasmid#70708PurposeHuman expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAGDepositorInsertmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationE782K, N968K and R1015H in SaCas9PromoterCAGAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only
