We narrowed to 9,355 results for: bli

1. SOX9-T2A-NLS-tdTomato-PGK-Puro

   Plasmid

   #89993

   Purpose

   Donor template for generation of SOX9-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AK2

   Plasmid

   #38829

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AK2 (AK2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pSG5-HA-ERR3 E275A,R316A

   Plasmid

   #107768

   Purpose

   Expresses a modified receptor with inactivating mutations in key ligand binding residues

   Depositor

   Rebecca Riggins

   Insert

   Estrogen-related receptor gamma (Esrrg Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E275A,R316A double mutant

   Promoter

   SV40

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV_puro_41584

   Plasmid

   #111630

   Purpose

   Complementation of Dicer_KO mESCs with wild type human DICER isoform

   Depositor

   Constance Ciaudo

   Insert

   FLAG-hsDicer (DICER1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG/HA-hBIG1

   Plasmid

   #226253

   Purpose

   Expresses N-terminally HA-tagged hBIG1 in mammalian cells

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LRRK2_1867_2136

   Plasmid

   #40408

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   GST-Tev and HisTev

   Mutation

   WT aa1867_2136

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEMB018 / LRRK2_BC_1847_2350

   Plasmid

   #40533

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Mutation

   WT; aa1847_2350

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. p5E-elavl3

   Plasmid

   #72640

   Purpose

   Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish

   Depositor

   Joshua Bonkowsky

   Insert

   elavl3 enhancer (elavl3 Zebrafish)
   

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CLK1

   Plasmid

   #39149

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CLK1 (CLK1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid

    #221158

    Purpose

    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Tags

    mGFP

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid

    #221159

    Purpose

    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Tags

    mScarletI

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-FLEX-ChR2-YFP

    Plasmid

    #110337

    Purpose

    ChR2-YFP reporter vector of iTango and Cal-Light system for AAV production

    Depositor

    Hyungbae Kwon

    Insert

    tetO-Flex-ChR2-YFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Chr2 H134R

    Promoter

    tetO

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-ABE-P48R-UGI

    Plasmid

    #161817

    Purpose

    Expresses ABE-P48R-UGI in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AK1

    Plasmid

    #39134

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK1 (AK1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. FLAG-hSRF (#482)

    Plasmid

    #78343

    Purpose

    Overexpression of human SRF ORF with a N-terminal FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    SRF (SRF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Human-Dysferlin-v1

    Plasmid

    #60217

    Purpose

    Human dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

    Depositor

    Jain Foundation

    Insert

    Dysferlin (DYSF Human)
    

    Use

    Gateway entry clone

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. BRD3

    Plasmid

    #38941

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD3 (BRD3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. AK5

    Plasmid

    #39136

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK5 (AK5 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCB301TOR-GFP

    Plasmid

    #164799

    Purpose

    Expression of GFP in oomycetes using Agrobacterium-mediated transformation

    Depositor

    Miaoying Tian

    Article

    Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

    Insert

    GFP
    

    Use

    Oomycete expression

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22-Nsp10-TEV-6His

    Plasmid

    #154957

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-6His

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. CFP-GIV-WT

    Plasmid

    #71544

    Purpose

    Mammalian expression of The CFP-tagged GIV-WT biosensors

    Depositor

    Pradipta Ghosh

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Tags

    CFP

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1 CARMA1

    Plasmid

    #44431

    Depositor

    Xin Lin

    Insert

    CARMA1 (CARD11 Human)
    

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Available Since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-2TK 14-3-3 sigma GST

    Plasmid

    #11944

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 sigma (SFN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. DUSP16

    Plasmid

    #38918

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DUSP16 (DUSP16 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pFLAG_MDA5∆2CARD

    Plasmid

    #216800

    Purpose

    Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    

    Expression

    Mammalian

    Mutation

    ∆1-287

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. K980

    Plasmid

    #129762

    Purpose

    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JMJD5

    Plasmid

    #39113

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD5 (KDM8 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pLEX307-TMPRSS11D-G418

    Plasmid

    #158477

    Purpose

    Constitutive lentiviral expression of human TMPRSS11D.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS11D (TMPRSS11D Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BRD3

    Plasmid

    #38940

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD3 (BRD3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

32. MSCV-mMDM2-IRES-GFP

    Plasmid

    #66954

    Purpose

    retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site

    Depositor

    Martine Roussel

    Insert

    Mdm2 (Mdm2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

    Plasmid

    #220508

    Purpose

    TGFb-inducible fluorescent reporter (TIE:EGFP)

    Depositor

    Leonard Zon

    Inserts

    TGFb inducible enhancer
    beta-globin minimal promoter
    SV40 PolyA signal
    

    Use

    Zebrafish expression (tol2)

    Tags

    EGFP

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

    Plasmid

    #64035

    Purpose

    Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs

    Depositor

    Karel Svoboda

    Insert

    hSyn-FAS-DO-ReaChR-mCitrine-WPRE
    

    Use

    AAV

    Tags

    ReaChR tagged with mCitrine

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

35. LACTB2

    Plasmid

    #39115

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    LACTB2 (LACTB2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pLJM1-4EBP1-T37A/T46A/Y54A/L59A

    Plasmid

    #136421

    Purpose

    Stable overexpression of elF4EBP1 T37A/T46A/Y54A/L59A

    Depositor

    Gabriel Leprivier

    Insert

    eIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    T37A/T46A/Y54A/L59A

    Promoter

    CMV

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GOSR2-mCherry

    Plasmid

    #209885

    Purpose

    Expresses human GOSR2 (GS27) fused to mChery in mammalian cells

    Depositor

    Geert van den Bogaart

    Insert

    GOSR2 (GOSR2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBJKS-mPar4

    Plasmid

    #53232

    Purpose

    mammalian expression of mouse Par4

    Depositor

    Shaun Coughlin

    Insert

    Par4 (F2rl3 Mouse)
    

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-17 (native signal peptide)

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

39. 4RCI

    Plasmid

    #64653

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    April 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pET28-Nsp14-TEV-6His

    Plasmid

    #154952

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-6His

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge