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pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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p5E_(-3kb)nrg1 type II

Plasmid

#199393

Purpose

pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

(-3kb)nrg1 type II

Use

Gateway donor vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_ngn1_LSE_C

Plasmid

#199395

Purpose

pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

LSE_C

Use

Gateway donor vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPEPX-Plac

Plasmid

#122632

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPEPY-Plac

Plasmid

#122634

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcNB2

Plasmid

#89242

Purpose

Resurfaced Cell-penetrating 5F7 nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB2 (5F7)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-GFP-HDEL

Plasmid

#21771

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 2001 Apr 2. 153(1):47-62.

Insert

Green Fluorescent Protein (GFP)

Tags

HDEL and preKar2

Expression

Yeast

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-A20 (OTU, aa 1-366)

Plasmid

#61584

Purpose

Expresses human A20 (OTU domain) in E. coli.

Depositor

David Komander

Article

Komander et al Biochem J. 2008 Jan 1;409(1):77-85.

Insert

A20 (TNFAIP3 Human)

Tags

GST-3C

Expression

Bacterial

Mutation

Deleted aa 367-790.

Promoter

tac

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly ZER1

Plasmid

#191540

Purpose

Protein expression of ZER1

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

ZER1 (ZER1 Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Promoter

T7

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MAP1LC3A

Plasmid

#25185

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubh67.001.121 (MAP1LC3A Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 9, 2010

Availability

Industry, Academic Institutions, and Nonprofits
LRT2B-AdGO

Plasmid

#136910

Purpose

Lentivirus expressing AdGO sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgAdGO

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pASR102

Plasmid

#122636

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pASR130

Plasmid

#122638

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcNB3

Plasmid

#89243

Purpose

Resurfaced Cell-penetrating B-lac nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB3 (B-Lac)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEW65: pQE-81L H3(K79M)

Plasmid

#124100

Purpose

H3K79M expression plasmid for norleucine supplementation

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Histone H3 (LOC108704296 Frog)

Expression

Bacterial

Mutation

K79M

Promoter

T5

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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PCx35IL-pET42

Plasmid

#42916

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 35 cytoplasmic loop

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 101-179

Promoter

T7

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-RNase HIII

Plasmid

#12683

Depositor

SeWon Suh

Article

Kwak et al Acta Crystallogr D Biol Crystallogr. 200

Insert

RNase HIII

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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CHKB (3FEG)

Plasmid

#25302

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CHKB:r35-s395 (CHKB Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids R35-S395

Available Since

Aug. 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits