We narrowed to 10,901 results for: Kars

1. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Use

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Psicheck2 PTPRN2 full length 3'UTR

   Plasmid

   #26994

   Depositor

   Joan Massague

   Article

   Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

   Insert

   PTPRN2 Full length 3' UTR (PTPRN2 Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

5. FUW-TetO-Nr5a1

   Plasmid

   #41081

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

   Insert

   Nr5a1 (Nr5a1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO + minimal CMV

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AIRAP 9: PSMD2.GST.V2.pcDNA3

   Plasmid

   #21799

   Depositor

   David Ron

   Article

   Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

   Insert

   PSMD2 (PSMD2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV_HaloK73T_cyto

   Plasmid

   #183687

   Purpose

   Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   Cyto Halo(K73T)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K73T in Halotag

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-mH6-Cas12i1

   Plasmid

   #120882

   Purpose

   Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNK6032

   Plasmid

   #219738

   Purpose

   MoClo-compatible Level 0 - promoter + 5U vector carrying promoter FMV

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

   Insert

   Figwort mosaic virus (FMV) promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   FMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1 shFEN

    Plasmid

    #17921

    Depositor

    Sheila Stewart

    Article

    Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

    Insert

    shFEN (FEN1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pJBL7056

    Plasmid

    #167225

    Purpose

    orthogonal T7 RNAP sfGFP reporter

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJCC_039 FnCas12a

    Plasmid

    #171666

    Purpose

    For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUb RxCas13d + RxCas13d guide RNA

    Plasmid

    #176303

    Purpose

    Expresses RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET11a-CA(A14C, E45C)

    Plasmid

    #79045

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    CA (A14C, E45C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A14C and E45C mutations in HIV-1 CA protein

    Promoter

    T7

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLD-puro-CcVA

    Plasmid

    #24588

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUb dPspCas13b + PspCas13b guide RNA

    Plasmid

    #176306

    Purpose

    Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    H133A, H1058A

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCK364.306

    Plasmid

    #192642

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFAP-Lyn-mCherry-pHluorin

    Plasmid

    #187980

    Purpose

    Membrane targeted cytoplasmic fluorescent pH sensor

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Lyn-mcherry-pHluorin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHSP70B-CP6-FCU1

    Plasmid

    #165882

    Purpose

    Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK869

    Plasmid

    #219737

    Purpose

    MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

    Insert

    promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtPD7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge